Monoclonal Anti-Rabbit IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in mouse
SIGMA/A2556 - clone RG-96, purified immunoglobulin, buffered aqueous glycerol solution
Synonym: Monoclonal Anti-Rabbit IgG (γ-chain specific)
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of human tonsil stained with Anti-Human IgG (whole molecule) antibody produced in Rabbit (Cat. No. I8635) followed by 1:100 Monoclonal Anti-Rabbit IgG (γ-chain specific)-Alkaline Phosphatase antibody produced in Mouse (Cat. No. A2556).
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with 1 μg/mL Anti-Actin, N-terminal antibody produced in Rabbit (Cat. No. A2103). The antibody was developed using Monoclonal Anti-Rabbit IgG (γ-chain specific)-Alkaline Phosphatase antibody produced in Mouse (Cat. No. A2556). Lanes 1. 1:200,000 antibody 2. 1:400,000 antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | RG-96, monoclonal |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous glycerol solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | wet ice |
| should not react with | goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken |
| species reactivity | rabbit |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:50,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 | |
| western blot: 1:200,000-1:400,000 using total cell extract of HeLa cells |
| Application: | Monoclonal Anti-Rabbit IgG (γ-chain specific)Alkaline Phosphatase antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting. |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Rabbit IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is a major class of immunoglobulin. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE. |
| General description: | The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep. |
| Physical form: | Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |