Angiotensin Converting Enzyme from porcine kidney
SIGMA/A2580 - lyophilized powder, ≥10 units/mg protein (Bradford)
Synonym: ACE; Peptidyl-dipeptidase A
CAS Number: 9015-82-1
MDL Number: MFCD00130325
Product Type: Chemical
| form | lyophilized powder |
| Quality Level | 200  |
| shipped in | dry ice |
| specific activity | ≥10 units/mg protein (Bradford) |
| storage temp. | −20°C |
| UniProt accession no. | A0A480IQM9  |
| Application: | Angiotensin converting enzyme from porcine kidney has been used in in vitro angiotensin-converting enzyme (ACE) inhibition assay. |
| Biochem/physiol Actions: | Angiotensin converting enzyme (ACE) catalyzes the conversion of angiotensin I to angiotensin II, which regulates the fluid-electrolyte balance and systemic blood pressure. ACE inhibits the vasodilator, bradykinin. ACE inhibitors are used to treat high blood pressure. |
| Biochem/physiol Actions: | Removes C-terminal dipeptides from susceptible substrates, e.g., angiotensin I and bradykinin. |
| General description: | ACE is a monomer with molecular weight of ~170 kDa pH range for activity: 7-8.5 Temperature optimum: 37 °C Zinc is required for activity Inhibitors: captopril, enalaprilat, lisinopril etc. (1-10 μM) |
| General description: | Angiotensin converting enzyme (ACE) is encoded by the gene, mRNA-decapping enzyme subunit 1 (DCP1). It is a zinc metalloprotease, which belongs to the M2 family. |
| Physical form: | Lyophilized powder containing Tris buffer salts. |
| Unit Definition: | One unit will produce 1.0 ·μmole of hippuric acid from Hippuryl-His-Leu per min in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37 °C. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥10 units/mg protein (Bradford) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.15.1 ( BRENDA | IUBMB ) |
| UNSPSC | 12352200 |