Acetylcholine chloride
SIGMA/A2661 - suitable for cell culture
Synonym: ACh
CAS Number: 60-31-1
Empirical Formula (Hill Notation): C7H16ClNO2
Molecular Weight: 181.66
EC Number: 200-468-8
MDL Number: MFCD00011698
Linear Formula: (CH3)3N+CH2CH2OCOCH3Cl-
Product Type: Chemical
| assay | ~99% |
| biological source | synthetic |
| form | powder |
| InChI | 1S/C7H16NO2.ClH/c1-7(9)10 |
| InChI key | JUGOREOARAHOCO-UHFFFAOYSA |
| mp | 146-150 °C (lit.) |
| Quality Level | 200  |
| shipped in | ambient |
| SMILES string | [Cl-].CC(=O)OCC[N+](C)(C) |
| solubility | water: 100 mg/mL, clear, colorless |
| storage temp. | room temp |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Acetylcholine chloride has been used in the preparation of acetylcholine, that is used as a common vehicle for iontophoresis of acetylcholine (ACh). It has also been used to treat aNS-1 derived neurons for cAMP (cyclic adenosine monophosphate) assay and also used to check their ability to influence G protein-coupled receptors (GPCRs). |
| Biochem/physiol Actions: | Acetylcholine chloride, injected at 20 mg/kg body weight, reduces mortality and plasma proinflammatory cytokines in mice with experimentally-induced sepsis . The cholinergic anti-inflammatory mechanism is probably mediated by interaction of acetylcholine with α7n cholinoreceptor on monocytes, macrophages, and neutrophils, which decreases the levels of proinflammatory cytokines such as TNF-α, IL-1β, and IL-6. |
| Biochem/physiol Actions: | Endogenous neurotransmitter at cholinergic synapses; amplifies action potential of the sarcolemma thereby inducing muscle contractions. |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ~99% |
| mp | 146-150 °C (lit.) |
| Storage Temp. | room temp |
| UNSPSC | 12352209 |