Monoclonal Anti-Albumin antibody produced in mouse
SIGMA/A2672 - clone HSA-9, ascites fluid
Synonym: Anti-AFP
MDL Number: MFCD00162390
Product Type: Chemical
ELISA Monoclonal Anti-Albumin antibody produced in Mouse, Clone: HSA-9 (Cat. No. A2672), followed by 1:500 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917), was serially diluted to detect Albumin by indirect ELISA.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HSA-9, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | catfish, turkey, chicken, hamster, rabbit, horse, donkey, cat, marmoset, pig, guinea pig, mouse, sheep, dog, pigeon, gibbon, bovine, goat |
| species reactivity | rhesus monkey, baboon, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:500 |
| UniProt accession no. | P02768  |
| Application: | Monoclonal Anti-Albumin antibody produced in mouse has been used in: • enzyme linked immunosorbent assay (ELISA) • immunoblotting • immunofluorescence |
| Application: | Western blot analysis of monkey retinal protein extracts were performed using the mouse monoclonal anti-albumin antibody at 1:20000 and overnight incubation at 4 degrees. Immunohistochemistry was performed on frozen monkey retina sections with the mouse monoclonal anti-albumin antibody at 1:1000. Uptake of albumin by LLC-PK1 cells was monitored by immunofluorescence using the mouse monoclonal anti-albumin antibody. Cells were first fixed in 4% formaldehyde the incubated with the antibody for 1 hour at room temperature. |
| Biochem/physiol Actions: | Albumin is a transport protein that binds a broad range of ligands such as fatty acids, bilirubin, hemin, drugs, amino acids and ions . Albumin may also function as a zinc carrier protein and consequently regulate physiological processes . Three major functions for serum albumin have been proposed: maintenance of osmotic pressure, transportation of a variety of substances and an endogenous source of amino acids. The primary sites of albumin degradation are not known, but the protein can be metabolized by almost every organ in the body. Determination of serum albumin levels is a widely used screening test in clinical medicine. A decrease in serum albumin levels may indicate disease states such as malnutrition, cirrhosis, nephrotic syndrome, diabetes, gastrointestinal and hepatic diseases, thermal burns and pulmonary disease. |
| Biochem/physiol Actions: | Serum albumins are implicated in the three major functions such as maintenance of osmotic pressure, transportation of a variety of substances and an endogenous source of amino acids. The primary sites of albumin degradation are not known, but the protein can be metabolized by almost every organ in the body. Determination of serum albumin levels is a widely used screening test in clinical medicine. A decrease in serum albumin levels may indicate disease states such as malnutrition, cirrhosis, nephrotic syndrome, diabetes, gastrointestinal and hepatic diseases, thermal burns and pulmonary disease. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Albumin, the major protein produced by liver cells, represents more than half of the total protein content of human serum. Many other body fluids also contain albumin. Three major functions for serum albumin have been proposed: maintenance of osmotic pressure, transportation of a variety of substances and an endogenous source of amino acids. The primary sites of albumin degradation are not known, but the protein can be metabolized by almost every organ in the body. Determination of serum albumin levels is a widely used screening test in clinical medicine. A decrease in serum albumin levels may indicate disease states such as malnutrition, cirrhosis, nephrotic syndrome, diabetes, gastrointestinal and hepatic diseases, thermal burns and pulmonary disease. |
| General description: | Monoclonal anti-Human Serum Albumin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| Immunogen: | Human platelets |
| Physical form: | The product is provided as ascites fluid with 0.1% sodium azide as a preservative. |
| Specificity: | The antibody is specific for human albumin in serum and plasma and recognizes an epitope sensitive to reduction commonly occurring in immunoblotting assays. No cross-reactivity is observed with bovine, cat, catfish, chicken, dog, donkey, gibbon, goat, guinea pig, hamster, horse, marmoset, mouse, pig, pigeon, rabbit, rat, sheep and turkey serum albumin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |