Aldolase from rabbit muscle
SIGMA/A2714 - lyophilized powder, ≥8.0 units/mg protein
Synonym: D-
CAS Number: 9024-52-6
EC Number: 232-781-0
MDL Number: MFCD00130453
Product Type: Chemical
biological source | rabbit muscle |
composition | Protein, ≥80% biuret |
foreign activity | glyceraldehyde-3-phosphat |
lactic dehydrogenase ≤0.03% | |
phosphoglucose isomerase ≤0.6% | |
pyruvate kinase ≤0.1% | |
triosephosphate isomerase ≤0.05% | |
form | lyophilized powder |
Quality Level | 300 |
specific activity | ≥8.0 units/mg protein |
storage temp. | −20°C |
Application: | Aldolase is used to convert fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, from rabbit muscle has been used for stereospecific deprotonation at DHAP C3 . Product A2714 is essentially sulfate-free and contains citrate buffer salts. |
Biochem/physiol Actions: | Aldolase is involved in gluconeogenesis, the Calvin cycle and glycolysis. Aldolase, from rabbit muscle, is a class I aldolase which forms covalent Schiff base intermediates. The active site of aldolase is in the center of the α/β 8 barrel fold . |
Packaging: | 100, 200, 500 units in poly bottle |
Physical form: | Essentially sulfate-free containing citrate buffer salts |
Unit Definition: | One unit will convert 1.0 μmole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per min at pH 7.4 at 25 °C. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
activity | specific activity: ≥8.0 units/mg protein |
Storage Temp. | −20°C |
Enzyme Commission (EC) Number | 4.1.2.13 ( BRENDA | IUBMB ) |
UNSPSC | 12352204 |