Monoclonal Anti-Na+/K+ ATPase (α Subunit) antibody produced in mouse
SIGMA/A276 - clone M7-PB-E9, ascites fluid
Synonym: Anti-CMT2DD; Anti-HOMGSMR2
MDL Number: MFCD02096998
Product Type: Chemical
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | M7-PB-E9, monoclonal |
| conjugate | unconjugated |
| isotype | IgG1 |
| mol wt | antigen ~110 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | rat, Xenopus |
| species reactivity | canine, chicken, human, sheep, pig, bovine, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunofluorescence: 1:20 | |
| immunohistochemistry (frozen sections): 1:100 | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1:500-1:5000 | |
| UniProt accession no. | P05023  |
| Application: | Monoclonal Anti- Na+/K+ ATPase ( α Subunit) antibody produced in mouse has been used in flow cytometry. |
| Biochem/physiol Actions: | The Na+/K+ ATPase is implicated in the maintenance of ion homeostasis for regular neuronal excitability, secondary transport and neurotransmitter uptake. ATPase Na+/K+ transporting subunit α 1 (ATP1A1) participates in both pumping and signaling functions. ATP1A1 regulates cellular transmembrane sodium and potassium ion gradients essential for the control of resting membrane potential. Additionally, nucleotide binding domain of ATP1A1 facilitates cellular signal transduction and cell growth. Deficiency of the gene leads to development of major depressive disorder (MDD). |
| Biochem/physiol Actions: | The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the α subunit, and an associated smaller glycoprotein, termed the β subunit. Studies indicate that there are three isoforms of the α subunit (α 1, α 2, α 3) and two isoforms of the β subunit (β 1 and β 2) encoded by two multigene families. The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The α 1 and β mRNAs are present in all cell types examined, whereas the α 2 and α 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The α subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The Na+/K+ ATPase (NKA) enzyme contains α, β and γ subunits. This enzyme is widely expressed. ATPase Na+/K+ transporting subunit α 1 (ATP1A1) is a principal catalytic subunit of NKA. It has a molecular mass of about 110kDa with 10 transmembrane segments. α-subunit has four different isoforms with tissue specific expression. α1 is primarily expressed in various tissues, including kidney, nerves and lung. While α2 is expressed in skeletal muscle and heart, α3 is found in the brain and α4 is apparently localized to testis and specifically to spermatozoa. ATP1A1 is encoded by the gene mapped to human chromosome 1p13-p11. |
| Immunogen: | sheep kidney α subunit of Na+/K+ ATPase. |
| Physical form: | solution containing 1 mg/ml BSA and 0.05% sodium azide |
| Target description: | Na+/K+ transporting ATPase subunit α1 is an ion transport pump critical for maintaining the gradient of Na and K ions across the plasma membrane. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |