Azocasein
SIGMA/A2765 - protease substrate
Synonym: Sulfanilamide-
CAS Number: 102110-74-7
MDL Number: MFCD00130592
Product Type: Chemical
ε (extinction coefficient) | ≥25 at 440 nm in 0.1 M NaOH at 1% |
biological source | bovine |
form | powder |
Quality Level | 200 |
solubility | water: 5 mg/mL, clear, orange to very deep orange |
storage temp. | 2-8°C |
technique(s) | ligand binding assay: suitable |
Application: | Azocasein has been used as a substrate for determination of protease activity. |
Application: | Azocasein is a nonspecific protease substrate. Hydrolysis of the casein releases the azo dye into the media where it is detected by absorbance at 440 nm. |
Application: | Azocasein is an inflammatory agent that is used to induce amyloid A amylooidosis in experimental animals. |
General description: | Azocasein is a chromogenic derivative of casein. Protease degrades azocasein to yield TCA-soluble azopeptides with high UV-absorbance. This azocasein assay is widely employed to estimate the protease production by bacterial fermentation on synthetic substrates from glucose and inorganic salts. |
Linkage: | View more information on azocasein protease assay at www.sigma-aldrich.com/enz |
Packaging: | 1, 5, 10, 25 g in glass bottle |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | 2-8°C |
UNSPSC | 12352204 |