Rabbit IgG−Agarose
SIGMA/A2909 - saline suspension
Synonym: IgG agarose beads
MDL Number: MFCD00165677
Product Type: Chemical
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| conjugate | agarose conjugate |
| extent of labeling | ≥5 mg per mL |
| form | saline suspension |
| matrix | cross-linked 4% beaded agarose |
| matrix activation | cyanogen bromide |
| matrix spacer | 1 atom |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| technique(s) | immunoprecipitation (IP): suitable |
| Application: | Rabbit IgG antibody (20 μl/ml) crosslinked to agarose beads was used to isolate RAT tagged proteins from whole cell extracts of 293T cells.Rabbit IgG crosslinked to agarose beads were used for purification of tagged protein from mammalian whole cell extracts at 0.5 to 1.0mg total protein to 10 μl of beads. |
| Application: | Rabbit IgG-Agarose has been used for immunoprecipitation and affinity purification assays. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgGs are glycoprotein antibodies that modulate several immune responses. IgG-Agarose is an immunoadsorbent that can be used to purify antibodies, remove species specific cross-reacting antibodies, or remove contaminating antibodies from an antiserum preparation. Characteristically, cross-reacting antibodies may be removed from an antiserum preparation using an equal resin volume of IgG-Agarose. However, the resin to antiserum ratio will vary with individual applications. Immunoglobulin G (IgG) is part of the immunoglobulin family and is a widely expressed serum antibody. It consists of a γ heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50 kDa and 25 kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Packaging: | 2, 5 mL in glass bottle |
| Physical form: | suspension in 0.5 M NaCl containing preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |