Acrylamide/Bis-acrylamide
SIGMA/A2917 - BioReagent, 19:1 (ratio), Molecular Biology
Synonym: Acrylamide/
MDL Number: MFCD00080848
Product Type: Chemical
| feed ratio | 19:1 (ratio) |
| foreign activity | DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected |
| form | powder |
| grade | Molecular Biology |
| InChI | 1S/C7H10N2O2.C3H5NO/c1-3- |
| InChI key | OCQZXMCGTAWGEQ-UHFFFAOYSA |
| product line | BioReagent |
| Quality Level | 200  |
| SMILES string | NC(=O)C=C.C=CC(=O)NCNC(=O |
| Application: | Acrylamide/Bis-acrylamide has been used: • in the preparation of urea polyacrylamide gel during a modified cross-linking affinity purification protocol (cCLAP) • in microsatellite genotyping and nonfluorescent elastin (ELN) (GT)n polymorphism genotyping • in polyacrylamide gel for separation of proteins in SDS-PAGE for western blotting |
| Application: | Ready to reconstitute dry powder blends are accurately pre-blended to produce a 40% (w/v) stock solution for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide. |
| Features and Benefits: | Eliminates the need to weigh toxic acrylamide and bis-acrylamide. |
| General description: | Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel. |
| Packaging: | 1 L in poly bottle |
| Packaging: | 100 mL in poly bottle |
| Preparation Note: | Reconstitute by slowly adding the indicated amount of distilled water to the container and dissolve contents. |
| Preparation Note: | To make up 100 ml of A2917, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution. For the 1 Liter size, use 630 mL of water. |
| Symbol |   GHS06,GHS08 |
| Signal word | Danger |
| Hazard statements | H301 - H312 + H332 - H315 - H317 - H319 - H340 - H350 - H361f - H372 |
| Precautionary statements | P202 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Codes | T |
| Risk Statements | 45-46-20/21-25-36/38-43-48/23/24/25-62 |
| Safety Statements | 53-26-36/37-45 |
| RIDADR | UN 2074 6.1 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41105319 |