Amphotericin B solution
SIGMA/A2942 - 250 μg/mL in deionized water, 0.1 μm filtered, BioReagent, suitable for cell culture
Synonym: Amphotericin; Fungizone
CAS Number: 1397-89-3
Empirical Formula (Hill Notation): C47H73NO17
Molecular Weight: 924.08
MDL Number: MFCD00877763
Linear Formula: C47H73NO17
Product Type: Chemical
antibiotic activity spectrum | fungi |
yeast | |
color | yellow |
concentration | 250 μg/mL in deionized water |
form | liquid |
InChI | 1S/C47H73NO17/c1-27-17-15 |
InChI key | APKFDSVGJQXUKY-INPOYWNPSA |
mode of action | cell membrane | interferes |
product line | BioReagent |
Quality Level | 200 |
shipped in | dry ice |
SMILES string | [H][C@]12C[C@@H](O[C@@H]3 |
sterility | 0.1 μm filtered |
storage temp. | −20°C |
technique(s) | cell culture | mammalian: suitable |
Application: | Amphotericin B solution has been used: • as an intraperitoneal injection for the treatment of Aspergillus fumigatus infection in mice • as a constituent of aspiration medium for the culture of Chinese Merino ewe ovaries • as a constituent of transport medium during the collection of equine corpora lutea • as a supplement in RPMI medium for the culture of the mouse-mouse B cell hybridoma 55-6 cell line |
Biochem/physiol Actions: | Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. that is clinically used to treat systemic fungal infections. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium. |
Biochem/physiol Actions: | Amphotericin B is most effective against fungi and yeast. It forms transmembrane channels by binding to sterols associated with membranes, thereby leading to the leakage of small molecules. Amphotericin B elicits toxicity despite being an effective antibiotic for treating mycoses. |
Caution: | Amphotericin B remains active for 3 days in culture at 37°C. |
Components: | This product is supplied as 250 μg/mL Amphotericin B in deionized water. It is a solution containing Amphotericin B, and the following components aimed to improve its solubility: sodium deoxycholate, sodium chloride and sodium phosphate. |
General description: | Amphotericin B is a macrocyclic lactone with a hydrophilic polyhydroxyl chain and a lipophilic polyene based hydrocarbon chain. |
Other Notes: | Keep container tightly closed in a dry and well-ventilated place. Light sensitive. |
Packaging: | 20,50,100ml |
Preparation Note: | It is not unusual for this material to form a precipitate in aqueous solutions. Mixing the solution will redissolve most of the precipitate but, if it does not fully resolubilize, the Amphotericin B will still be suitable for use. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
UNSPSC | 51101500 |