Monoclonal Anti-Alkaline Phosphatase, Human Placental antibody produced in mouse
SIGMA/A2951 - clone 8B6, ascites fluid
Synonym: Monoclonal Anti-Alkaline Phosphatase, Human Placental; Anti-Phosphatase, Alkaline, Human Placental; PLAP
MDL Number: MFCD00162106
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human placenta tissue sections were stained with 1:8,000 Monoclonal Anti-Alkaline Phosphatase, Human Placental antibody produced in Mouse, Clone: 8B6 (Cat. No. A2951) followed by 10 μg/mL Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat (Cat. No. B7151) and by 10 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 8B6, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:4,000 using human placenta | |
| immunohistochemistry (frozen sections): suitable | |
| indirect ELISA: suitable using whole cells | |
| radioimmunoassay: suitable using radio-immunolocalization of xenografts of human cell lines expressing PLAP in nude mice2 | |
| western blot: suitable | |
| UniProt accession no. | P05187  |
| Application: | Anti-Alkaline phosphatase human placental antibody coupled to agarose was packed in a column and used to purify alkaline-phosphatase conjugated FGF receptor out of conditioned media from COS-7 cells. Column was washed with 1M NaCl. |
| Application: | Cultured pancreatic islet cells were fixed with cold 4% paraformaldhyde at room temperature for 10 minutes and incubated with mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody at 1:500. Mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody was used to detect a unique molecular probe that consisted of the FGFR extracellular domain expressed as a chimera with human PLAP. |
| Application: | Monoclonal Anti-Alkaline Phosphatase, Human Placental antibody produced in mouse has been used in: • enzyme-linked immunosorbent assay (ELISA) • immunocytochemistry • radio-immunolocalizatio • western blot • immunofluorescence • immunohistochemistry |
| Biochem/physiol Actions: | Placental alkaline phosphatase (PLAP) or ALPP is a hydrolase, which is involved in the hydrolysis of phosphomonoesters. It has been shown to be a biomarker for ovarian cancer. The protein is expressed in carcinomas such as ovarian adenocarcinoma, dysgerminoma, serous cystadenocarcinoma and undifferentiated carcinoma. Although hPLAP has been analyzed as a potential tumor marker, it has not been widely used in the routine clinical laboratory because of its low overall specificity due to heat-inactivation, cross-reactivity with intestinal AP and |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | In SDS gels, the product reacts with both Regan and Nagao isozymes of human placental alkaline phosphatase (hPLAP, 130 kDa, 67/130 kDa). By RIA, the antibody binds to hPLAP with an affinity constant of 5 × 109 LM-1. It does not react with PLAP-like enzymes. |
| General description: | Monoclonal Anti-Human Placental Alkaline Phosphatase (mouse IgG2a isotype) is derived from the 8B6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Placental alkaline phosphatase (PLAP) or ALPP is a phosphomonoesterase. It is expressed in the placenta and endometrium. The PLAP gene is localized on human chromosome 2q37.1. Small amounts of hPLAP are found in the endocervix, fallopian tubes and lung. Very small amounts of heat-stable AP resembling hPLAP (hPLAPlike AP) are expressed in the testis, thymus and in rare colon epithelial cells. |
| Immunogen: | Human epidermoid carcinoma cell line expressing placental alkaline phosphatase. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |