Anti-ATG4B antibody produced in rabbit
SIGMA/A2981 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-APG4B; Anti-AUT-like 1 cysteine endopeptidase; Anti-AUTL1; Anti-Autophagin-1; Anti-
MDL Number: MFCD09753846
Product Type: Chemical
Immunohistochemistry Anti-Atg4B: Cat. No. A2981: Immunoperoxidase staining of Anti-Atg4B in formalin fixed, paraffin-embedded rat brain at 1.3 μg/mL.
Immunohistochemistry Anti-Atg4B: Cat. No. A2981: Immunoperoxidase staining of Anti-Atg4B in formalin fixed, paraffin-embedded rat brain at 1.3 μg/mL.
Immunohistochemistry Anti-Atg4B: Cat. No. A2981: Immunoperoxidase staining of Anti-Atg4B in formalin fixed, paraffin-embedded mouse brain at 2.6 μg/mL.
Immunohistochemistry Anti-Atg4B: Cat. No. A2981: Immunoperoxidase staining of Anti-Atg4B in formalin fixed, paraffin-embedded mouse brain at 2.6 μg/mL.
Immunofluorescence HeLa cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 1:200 Rabbit Anti-ATG4B (Cat. No. A2981). The antibody was developed using Goat Anti-Rabbit IgG, ATTO-488 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Whole mouse 3T3 cell extract was separated on SDS-PAGE and blotted with Rabbit Anti-ATG4B (Cat. No. A2981). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1: Antibody 1:250 2: Antibody 1:500 3: Antibody 1:500 + ATG4B immunizing peptide (human 6-22)
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~44 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 using biotin/ExtaAvidin®-Peroxidase staining of heat-retrieved human cerebellum sections |
| western blot: 1:125-1:500 using whole extract of 3T3 cells | |
| western blot: 1:500-1:1,000 using whole extract of rat brain | |
| UniProt accession no. | Q9Y4P1  |
| Application: | Anti-ATG4B antibody produced in rabbit has been used in • western blotting • immunohistochemistry |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) Western Blotting (1 paper) |
| Biochem/physiol Actions: | ATG4B (autophagy-related 4B cysteine peptidase) participates in the modulation of autophagy in mammalian cells. It is also involved in light chain 3 (LC3) lipidation. ATG4B is responsible for the processing of the mammalian Atg8 homologues: Golgi-associated ATPase enhancer of 16 kDa (GATE16), gamma-aminobutyric-acid-t |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | ATG4B (autophagy-related 4B cysteine peptidase), a mammalian orthologue of yeast Atg4, is a cysteine protease. It is widely expressed in human and rat tissues, being most abundant in the brain. This gene is mapped to human chromosome 2q37. |
| General description: | ATG4B is a member of the C-54 family of cysteine proteases that is involved in cell degradation through autophagy. |
| Immunogen: | synthetic peptide corresponding to amino acids 6-22 of human ATG4B, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in mouse. |
| Legal Information: | ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |