Anti-Human IgE−Alkaline Phosphatase antibody, Mouse monoclonal
SIGMA/A3076 - clone GE-1, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Human IgE; Monoclonal Anti-Human IgE−Alkaline Phosphatase antibody produced in mouse
Product Type: Chemical
ELISA Monoclonal Anti-Human IgE-Alkaline Phosphatase antibody produced in Mouse, Clone: GE-1 (Cat. No. A3076) was serially diluted to detect Human IgE by direct ELISA.
| antibody form | purified from hybridoma cell culture |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | GE-1, monoclonal |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous solution |
| isotype | IgG2b |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:6,000 |
| dot blot: 1:1,000 |
| Analysis Note: | The antibody is tested for reactivity against myeloma IgE and allergy-elevated IgE. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Application: | Mouse monoclonal anti-human IgE-alkaline phosphatase antibody has been used for : • immunoblot assays • direct ELISA • dot blot |
| Application: | To confirm that a chimaeric monoclonal antibody had episolon chain, an elisa was performed using monoclonal anti-human IgE antibody (clone GE1) at a 1:1000 dilution. |
| Biochem/physiol Actions: | IgEs are glycoprotein antibodies that regulate immunological responses to allergies and infections. These immunoglobulins have been implicated in mediating Type I hypersensitivity reactions. Elevated IgE levels have been associated with hyper IgE syndrome and allergic asthma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Binds human IgE; does not bind human IgG, IgA or IgM. |
| General description: | IgEs are glycoprotein antibodies that regulate immunological responses to allergies and infections. These immunoglobulins have been implicated in mediating Type I hypersensitivity reactions. Elevated IgE levels have been associated with hyper IgE syndrome and allergic asthma. |
| General description: | Monoclonal Anti-Human IgE (mouse IgG2b isotype) is derived from the GE-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| Physical form: | Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |