Avidin–Peroxidase
SIGMA/A3151 - lyophilized powder
Synonym: Avidin-POD
MDL Number: MFCD00130587
Product Type: Chemical
| biological source | avidin from egg white |
| enzyme from horseradish | |
| composition | Protein, ≥70% E |
| conjugate | peroxidase conjugate |
| extent of labeling | 0.7-2.0 mol peroxidase per mol avidin |
| form | lyophilized powder |
| Quality Level | 200  |
| solubility | H2O: soluble 1 mg/mL |
| PBS: soluble | |
| storage temp. | −20°C |
| technique(s) | direct ELISA: 1:50,000 |
| Application: | Avidin-Peroxidase has been used for detecting anti-5-bromodeoxyuridine (anti-BrdU) binding and OVA-IgG1. |
| Application: | To detect Ga1-deficient IgA1 antibodies in mouse serum, ELISA assays were performed using sera which was incubated with biotinylated HAA, which binds IgA1, in 96-well plates. HRP-conjugated avidin was then incubated with the sera for 40 minutes at 37 degrees. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities. |
| Physical form: | Lyophilized powder containing citrate buffer. |
| Preparation Note: | Labeled with Type VI peroxidase. Coupled by a modification of the method of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |