Bovine Serum Albumin
SIGMA/A3156 - lyophilized powder, γ-irradiated, Globulin Free, BioXtra, suitable for cell culture
Synonym: Albumin bovine serum; BSA; Bovine albumin
CAS Number: 9048-46-8
EC Number: 232-936-2
MDL Number: MFCD00130384
Product Type: Chemical
assay | ≥99% (agarose gel electrophoresis) |
biological source | bovine |
form | lyophilized powder |
impurities | ≤10% water (Karl Fischer) |
mol wt | ~66 kDa |
origin | USA origin |
pH | 4.8-7.5 |
product line | BioXtra |
purified by | cold ethanol and heatshock fractionation |
Quality Level | 200 |
shipped in | ambient |
solubility | water: soluble (5g/50ml) |
sterility | γ-irradiated |
storage temp. | 2-8°C |
technique(s) | cell culture | mammalian: suitable |
UniProt accession no. | P02769 |
Application: | Bovine serum albumin has been used: • to prevent sticking of the chromosome to the glass micropipette for elasticity measurements using aspiration • to block lung homogenates in the plate for mucin protein enzyme-linked immunosorbent assay • in alginate solution as a protein model for drug release kinetics investigation |
Biochem/physiol Actions: | Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids. Albumin turnover is seen in infants with iron deficiency anemia. BSA acts as a vital constituent in the cell culture media and favors human embryonic stem cells (hESC) differentiation. |
Biochem/physiol Actions: | Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal. |
Biochem/physiol Actions: | Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies. |
General description: | Bovine serum albumin (BSA) is a 66 kDa protein consisting of three domains with two subdomains under each. It is a α-helical, globular and non-glycosylated protein. Albumin is the most abundant plasma protein in humans. |
General description: | BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group. |
Preparation Note: | Purified by a combination of heat shock and ethanol fractionation |
Preparation Note: | Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥99% (agarose gel electrophoresis) |
Storage Temp. | 2-8°C |
UNSPSC | 12352202 |