α-Amylase from porcine pancreas
SIGMA/A3176 - Type VI-B, ≥5 units/mg solid
Synonym: alpha-Amylase; β-N-acetylglucosaminidase porcine placenta; PPA; al1,4 glucan-
EC Number: 232-565-6
MDL Number: MFCD00081319
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A3176-1MU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A3176-500KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A3176-2.5MU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A3176-5MU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A3176-10MU
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
| application(s) | life science and biopharma |
| biological source | Porcine pancreas |
| concentration | ~90% (lactose) |
| form | powder |
| greener alternative category | Enabling  , |
| greener alternative product characteristics | Waste Prevention Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| Quality Level | 200 |
| specific activity | ≥5 units/mg solid |
| storage temp. | 2-8°C |
| suitability | suitable for hydrolysis, synthesis of oligosaccharides and polysaccharides, and sugar modification |
| sustainability | Greener Alternative Product |
| technique(s) | activity assay: suitable |
| type | Type VI-B |
| Application: | α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. α-Amylase, from Sigma, has been used in various plant studies, such as metabolism studies in Arabidopsis . α-Amylase, from porcine pancreas, has been used to remove starch during the study of starch metabolism and ripening in tomatoes . |
| Biochem/physiol Actions: | α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. α -Amylase, from porcine pancreas, is a glycoprotein that consists of a single polypeptide chain of approximately 475 residues containing 2 SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. The pH optimum is at 7. |
| General description: | Molecular mass: 51-54 kDa. α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. Chloride ions are necessary for activity and stability. The pH range for activity is 5.5 to 8.0, with the pH optimum at 7. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles . |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Packaging: | 500000, 1000000, 2500000, 5000000, 10000000 units in poly bottle |
| Packaging: | Package size based on α-amylase activity |
| Quality: | Contains lactose |
| Unit Definition: | One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Hazard Codes | Xn |
| Risk Statements | 42 |
| Safety Statements | 22-24-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥5 units/mg solid |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |