Anti-ATG3 antibody produced in rabbit
SIGMA/A3231 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-APG3; Anti-APG3L; Anti-Autophagy related 3 homolog (S. cerevisiae); Anti-DKFZp564M1178; Anti-FLJ22125; Anti-MGC15201; Anti-PC3-96
MDL Number: MFCD09753844
Product Type: Chemical
Immunoblotting Whole human HeLa cell extract was separated on SDS-PAGE and blotted with Rabbit Anti-ATG3 (Cat. No. A3231). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1: Antibody 2 μg/mL 2: Antibody 1 μg/mL 3: Antibody 1 μg/mL + ATG3 immunizing peptide (human 17-32)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~36 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μL using rat NRK cell lysate |
| western blot: 2-4 μg/mL using whole extracts of human HeLa and mouse 3T3 cells | |
| UniProt accession no. | Q9NT62  |
| Application: | Anti-ATG3 antibody produced in rabbit has been used in western blotting and immunoprecipitation. |
| Biochem/physiol Actions: | Autophagy-related protein 3 (ATG3) is involved in the formation of the autophagosome. the mammalian homolog of yeast Apg3p/Aut1p, is an E2-like enzyme that catalyzes the conjugation reaction between Atg8 and phosphatidylethanolamine (PE). Golgi-associated ATPase enhancer of 16 kDa (GATE-16), GABAA receptor-associated protein (GABARAP), and microtubule-associated protein light chain 3 (MAP-LC3) are substrates for Atg3. MAP-LC3 is the preferred substrate. Atg3 interacts with Atg7 to form an E1-E2 complex, and with Atg12, which is a substrate for Atg7 but not for Atg3. Moreover, overexpression of Atg3 facilitates the formation of the Atg12-Atg5 conjugate. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | ATG3 is a member of the autophagy-related protein family and is involved in the formation of the autophagosome. |
| General description: | Autophagy-related protein 3 (ATG3) is a member of the autophagy-related protein family. It is a mammalian homolog of yeast Apg3p/ Atg3 is ubiquitously expressed in human tissues. |
| Immunogen: | synthetic peptide corresponding to amino acids 17-32 of human Atg3, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |