α-Amylase from Bacillus licheniformis
SIGMA/A3403 - Type XII-A, saline solution, ≥500 units/mg protein (biuret)
Synonym: Termamyl® 2X; 1,4-α-D-Glucan-glucanohydrolase
CAS Number: 9000-85-5
MDL Number: MFCD00081319
Product Type: Chemical
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
| form | saline solution |
| greener alternative category | Enabling  , |
| greener alternative product characteristics | Waste Prevention Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| mol wt | 57.6 kDa |
| Quality Level | 200 |
| specific activity | ≥500 units/mg protein (biuret) |
| storage temp. | 2-8°C |
| sustainability | Greener Alternative Product |
| type | Type XII-A |
| Application: | α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. Product A3403 is from Bacillus licheniformis and is type XII-A. α-Amylase, from Sigma, has been used in various plant studies, such as metabolism studies in Arabidopsis . |
| Biochem/physiol Actions: | α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. |
| Caution: | Reported to be heat stable at temperatures as high as ∼90 °C. |
| General description: | α-Amylase is a member of the endo-amylase family. Its molecular weight is 57.6 kDa. α-Amylase is composed of three functional domains namely A, B and C. Domain A is a barrel-shaped (β/α)8 structure and is the largest domain. Domain B is linked to domain A via disulfide bond and is present between domain A and C. Domain C is composed of β-sheets and is connected to domain A via a polypeptide chain. We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles . |
| Legal Information: | A product of Novozyme Corp. |
| Legal Information: | Termamyl is a registered trademark of Novozymes Corp. |
| Physical form: | Aqueous solution containing sodium chloride and sucrose |
| Unit Definition: | One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P342 + P311 |
| Hazard Codes | Xn |
| Risk Statements | 42 |
| Safety Statements | 23-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥500 units/mg protein (biuret) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |