Achromopeptidase from bacteria
SIGMA/A3422 - partially purified powder, ≥20,000 units/mg solid
Synonym: ACP; Achromopeptidase
MDL Number: MFCD00130333
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A3422-25KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A3422-50KU
| foreign activity | Collagenase, present |
| form | partially purified powder |
| mol wt | 27 kDa |
| Quality Level | 200  |
| specific activity | ≥20,000 units/mg solid |
| storage temp. | −20°C |
| Application: | Achromopeptidase from bacteria may be used: • in the homogenization step for genomic DNA extraction from microbial biofilm • in the lysis of gram-positive strains( • for DNA extraction from Nocardia strains |
| Application: | Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections. Product A3422 is from bacteria and is a partially purified powder containing lactose. |
| Biochem/physiol Actions: | Achromopeptidase is a bacteriolytic enzyme and belongs to serine protease family. It is effective for the lysis of gram positive bacteria compared to lysozyme. Achromopeptidase activity is inhibited in the presence of heavy metals. Achromopeptidase based inhibition assay may be useful for screening the presence of heavy metals. |
| Biochem/physiol Actions: | Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 °C. |
| Other Notes: | One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C. |
| Physical form: | Partially purified powder. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P342 + P311 |
| Hazard Codes | Xn |
| Risk Statements | 42 |
| Safety Statements | 22-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥20,000 units/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.21.50 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |