Anti-ApoER2 antibody produced in rabbit
SIGMA/A3481 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Apolipoprotein E Receptor 2; Anti-LRP8; Anti-Low Density Lipoprotein Receptor-Related Protein 8
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-ApoER2 antibody produced in Rabbit (Cat. No. A3481) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing ApoER2 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:2,000.
Immunoblotting Whole cell lysates were separated on SDS-PAGE and blotted with Anti-ApoER2 (Cat. No. A3481). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and chemiluminescent substrate. Antibody dilution 1:2,000 Lanes 1,2: HEK293 cells co-transfected with human ApoER2 2: wt HEK293 cells (untransfected) 3: Inhibition with ApoER2 (human 928-945) peptide (20 μg/mL)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~105 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.5-1 μg/mL using HEK-293T cells expressing human ApoER2 |
| UniProt accession no. | Q14114  |
| Application: | Anti-ApoER2 antibody produced in rabbit has been used in western blot analysis. |
| Biochem/physiol Actions: | Apolipoprotein E receptor 2 (ApoER2, also known as LRP8) play an important role in the endocytosis of plasma lipoproteins, including ApoE and cholesterol metabolism. In the central nervous system, ApoER2 mediates various neuronal signaling mechanisms during neuronal development, affecting neurite outgrowth, synapse formation and neuronal migration, and neuronal survival in response to acute damage. ApoER2 plays a central role in the reelin/Dab1 signaling pathway that controls neuronal positioning during brain development as well as long-term potentiation (LTP) in the adult brain. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Apolipoprotein E receptor 2 (ApoER2, also known as LRP8) is expressed predominantly in the brain and placenta. This gene is located on human chromosome 1p32. |
| General description: | The low-density lipoprotein receptor-related protein 8 (LRP8) or ApoER2 is a member of the LDL receptor family which includes LDLR and VLDLR. These proteins are involved in the uptake of lipid rich proteins into cells and cell signaling. ApoER2 assists in endocytosis and signal transduction. |
| Immunogen: | synthetic peptide corresponding to amino acids 928-945 located near the C-terminus of human ApoER2, conjugated to KLH. This sequence is identical in mouse ApoER2. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Rabbit anti-ApoER2 antibody recognizes human ApoER2 by immunoblotting (approx. 105kDa). Staining of the ApoER2 band in immunoblotting is specifically inhibited by the ApoER2 immunizing peptide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |