Achromopeptidase from bacteria
SIGMA/A3547 - lyophilized powder, ≥1,000 units/mg solid
Synonym: ACP; Lysyl endopeptidase
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A3547-1MU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A3547-500KU
| application(s) | diagnostic assay manufacturing |
| biological source | bacterial ( |
| form | lyophilized powder |
| mol wt | 27 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| specific activity | ≥1,000 units/mg solid |
| storage temp. | −20°C |
| suitability | suitable for cell lysis |
| technique(s) | cell based assay: suitable |
| Application: | Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections. |
| Biochem/physiol Actions: | Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 °C. |
| Biochem/physiol Actions: | Achromopeptidase is a lysyl endopeptidase with a MW of ~27 kDa. It is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. pH Optimum for activity: pH 8.5 - 9 Approximately 500-1,500 un/ml achromopetidase can be used to lyse cells at a density of OD600=0.6 over 2 hours at 37 °C. |
| Other Notes: | One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C. |
| Packaging: | 100000 units in poly bottle |
| Packaging: | 500000, 1000000 units in glass bottle |
| Physical form: | crude powder |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P342 + P311 |
| Hazard Codes | Xn |
| Risk Statements | 42 |
| Safety Statements | 22-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| activity | specific activity: ≥1,000 units/mg solid |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |