Acrylamide
SIGMA/A3553 - suitable for electrophoresis, ≥99% (HPLC), powder
Synonym: 2-Propenamide; Polyacrylamide; acrylic amide; akrylamid; 2-Propenamide; Acrylic acid amide
CAS Number: 79-06-1
Empirical Formula (Hill Notation): C3H5NO
Molecular Weight: 71.08
EC Number: 201-173-7
MDL Number: MFCD00008032
Linear Formula: CH2=CHCONH2
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A3553-1KG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A3553-500G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A3553-100G
| assay | ≥99% (HPLC) |
| bp | 125 °C/25 mmHg (lit.) |
| cation traces | Fe: ≤1 ppm |
| heavy metals (as Pb): ≤5 ppm | |
| Mg: ≤3 ppm | |
| conductivity | ≤10 μmho (35% solution) |
| form | powder |
| impurities | ≤0.005% Acrylic acid |
| ≤0.005% Insoluble in water | |
| InChI | 1S/C3H5NO/c1-2-3(4)5/h2H, |
| InChI key | HRPVXLWXLXDGHG-UHFFFAOYSA |
| mp | 82-86 °C (lit.) |
| Quality Level | 200  |
| SMILES string | NC(=O)C=C |
| solubility | water: soluble 2.5 g/10 mL, clear, colorless |
| technique(s) | electrophoresis: suitable |
| vapor density | 2.45 (vs air) |
| vapor pressure | 0.03 mmHg ( 40 °C) |
| Application: | Acrylamide has been used in the preparation of polyacrylamide gels. |
| General description: | Acrylamide functions as a quencher of tryptophan fluorescence. Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels, which is used to separate protein and other biomolecules. Bis-acrylamide is essential for crosslinking and acrylamide is required for the polymerization of monomers. Acrylamide alkylates SH groups of cysteine and ε-NH2 lysine residues of proteins during electrophoresis. |
| Other Notes: | This substance has been tested against several types of hand protection for CE compliance. See Related Products to find the recommended gloves for handling this product. |
| Packaging: | 1 kg in glass bottle |
| Packaging: | 100, 500 g in glass bottle |
| Symbol |   GHS06,GHS08 |
| Signal word | Danger |
| Hazard statements | H301 - H312 + H332 - H315 - H317 - H319 - H340 - H350 - H361f - H372 |
| Precautionary statements | P201 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Codes | T |
| Risk Statements | 45-46-20/21-25-36/38-43-48/23/24/25-62 |
| Safety Statements | 53-45 |
| RIDADR | UN 2074 6.1 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | 280.4 °F - closed cup |
| Flash Point(C) | 138 °C - closed cup |
| Purity | ≥99% (HPLC) |
| bp | 125 °C/25 mmHg (lit.) |
| mp | 82-86 °C (lit.) |
| UNSPSC | 41105319 |