Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–Alkaline Phosphatase antibody produced in sheep
SIGMA/A3563 - affinity isolated antibody, buffered aqueous glycerol solution
MDL Number: MFCD00162640
Product Type: Chemical
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| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | sheep |
| clone | polyclonal |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous glycerol solution |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:30,000 |
| dot blot: 1:30,000 | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 | |
| western blot: 1:30,000 |
| Application: | Anti-Mouse IgG (whole molecule) F(ab’)2 fragment-Alkaline Phosphatase antibody is suitable for use in direct ELISA (1:30,000), western blot (1:30,000), and immunohistochemistry (at 1:50 dilution in formalin-fixed, paraffin-embedded sections). The antibody may also be used for dot blot and immunoblot assays using a working dilution of 1:30,000. |
| Application: | ELISAs using alkaline phosphatase conjugated sheep anti-mouse IgG whole molecule antibody were performed to measure the antibody response in the sera of mice immunixed to T. crassiceps. 1:1000 at 37 degrees for one hour and developed using p-nitrophenyl (Sigma). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice Sheep anti-mouse IgG (whole molecule) F(ab’)2 fragment is specific for the F(ab’)2 of mouse IgG. The antibody preparation consists only of the F(ab’)2 fragment of sheep IgG as determined by SDS-PAGE. No contamination with sheep IgG whole molecule is observed. |
| Immunogen: | Mouse IgG |
| Physical form: | Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |