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Acrylamide/Bis-acrylamide, 30% solution

SIGMA/A3574 - BioReagent, suitable for electrophoresis, 29:1

Synonym: 30% Acrylamide/Bisacrylamide 29:1; Polyacrylamide solution

MDL Number: MFCD00080848
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-A3574-100ML 100 mL
$94.30
1/EA
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45-A3574-5X100ML 100 mL
$165.00
5/EA
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45-A3574-5L 5 L
$563.00
1/EA
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feed ratio 29:1
foreign activity Protease, none detected
form liquid
InChI 1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)
InChI key OCQZXMCGTAWGEQ-UHFFFAOYSA-N
product line BioReagent
Quality Level 200 
SMILES string NC(=O)C=C.C=CC(=O)NCNC(=O)C=C
sterility 0.2 μm filtered
storage temp. 2-8°C
suitability suitable for electrophoresis
technique(s) electrophoresis: suitable
Application: Acrylamide/Bis-acrylamide, 30% solution has been used as a constituent in the preparation of 4% stacking gel and 10% resolving gel during sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel electrophoresis. It has also been used as a precursor for polyacrylamide lid fabrication by photopatterning methods.
Application: These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.
General description: Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.
Preparation Note: Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.
Storage Temp. 2-8°C; 2-8°C
UNSPSC 41105319

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