Anti-ATG3 (C-terminal) antibody produced in rabbit
SIGMA/A3606 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-APG3; Anti-APG3L; Anti-Autophagy related 3 homolog (S. cerevisiae); Anti-DKFZp564M1178; Anti-FLJ22125; Anti-MGC15201; Anti-PC3-96
MDL Number: MFCD09753845
Product Type: Chemical
Immunoprecipitation Rabbit Anti-ATG3 (C-terminal) antibody (Cat. No. A3606) was used to immunoprecipitate ATG3 from rat NRK (Lanes 1-3) and mouse 3T3 (Lanes 4-6) cell lysates Lanes 1,4: Antibody 2 μL 2,5: Antibody 1 μL 3,6: Non-relevant antibody Detection antibody: Rabbit Anti-ATG3 antibody (Cat. No. A3231)
Immunohistochemistry Formalin fixed, paraffin-embedded Human cerebellum tissue sections were stained with 1:50 Anti-ATG3 (C-terminal) antibody produced in Rabbit (Cat. No. A3606), followed by 20 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~36 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 using biotin/ExtrAvidin®-peroxidase staining of heat-retrieved sections of human cerebellum |
| immunoprecipitation (IP): 2-4 μL using rat NRK and mouse 3T3 cell lysates | |
| UniProt accession no. | Q9NT62  |
| Application: | Rabbit Anti-ATG3 (C-terminal) antibody can be used for immunohistochemistry (1:50 -1:100) and immunoprecipitation (2-4μl) assays. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Rabbit Anti-ATG3 (C-terminal) antibody recognizes human, rat, and mouse Atg3 by immunoprecipitation (approx. 36kDa) and immunohistochemistry. Detection of the Atg3 staining by immunohistochemistry is specifically inhibited with the immunizing peptide. |
| Immunogen: | synthetic peptide corresponding to amino acids 298-314 of human ATG3, conjugated to KLH via an N-terminal cysteine residue. |
| Legal Information: | ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Target description: | ATG3 is a member of the autophagy-related protein family and is involved in the formation of the autophagosome. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |