Apoferritin from equine spleen
SIGMA/A3641 - 0.2 μm filtered
CAS Number: 9013-31-4
MDL Number: MFCD00081365
Product Type: Chemical
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| color | clear to slightly hazy, solution |
| form | liquid |
| mol wt | major subunit ML 19,889 |
| minor subunit MH 22,200 | |
| native ~481.2 kDa (24 subunits, approx. 20 kDa each) | |
| Quality Level | 200  |
| sterility | 0.2 μm filtered |
| storage temp. | 2-8°C |
| Application: | Apoferritin from equine spleen has been used: • to determine its partition coefficients in phase systems • in NaCl for iron loading of cultured cells • in in situ liquid scanning transmission electron microscope for imaging the interface of biology and nanotechnology |
| Biochem/physiol Actions: | This protein shell of ferritin lacking iron is widely used for the calibration of gel filtration columns and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. It is very abundant in beta-cells of the pancreas where it acts as an anti-oxidant. When added to cultured endothelial cells apoferritin is taken up in a dose-responsive manner and appears to protect the cells from oxidant-mediated cytolysis. |
| Physical form: | Solution in 0.135 M sodium chloride. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |