Azurin
SIGMA/A3672 - from Pseudomonas aeruginosa, lyophilized powder
CAS Number: 12284-43-4
EC Number: 235-551-8
MDL Number: MFCD00130594
Product Type: Chemical
SDS-PAGE Azurin (Cat. No. A3672) was assessed on SDS-PAGE.
| biological source | Pseudomonas aeruginosa |
| composition | Protein, ≥65% Lowry |
| concentration | ≥65.0% (Lowry) |
| form | lyophilized powder |
| Quality Level | 200  |
| solubility | water: soluble 1—1.1 mg/mL, clear, blue (light blue to blue) |
| storage temp. | −20°C |
| technique(s) | toxicology assay: suitable |
| UniProt accession no. | P00282  |
| Application: | Azurin has been used: • in the cytotoxicity and cell viability studies in human osteosarcoma cell line • for the functionalization of silicon nitride cantilevers for interaction studies • for coating gold surface and insulating functionalized oxide surfaces of silicon oxide and mica |
| Biochem/physiol Actions: | Azurin acts as an electron donor for nitrite reductase in bacterial denitrification process. It exhibits anticancer activity as it hampers various independent signaling pathways associated with cancer progression. It binds to tumor suppressor protein p53 and induces cancer cell apoptosis or stalls cancer cell growth. Azurin disrupts angiogenesis by reducing the activity of VEGFR-2tyrosine kinase thereby inhibiting tumor growth. It has been observed to show cytotoxicity in human breast cancer cells and human melanoma cells. |
| Biochem/physiol Actions: | Azurin is a metalloprotein in the family of cupredoxins. It preferentially enters cancer cells over normal cells and induces apoptosis. Azurin has structural similarities to ephrinB2, and in fact binds the ephrin receptor tyrosine kinase EphB2 to initiate cell signaling that is involved in cancer progression. Azurin inhibits autophosphorlyation of the EphB2 tyrosine residue, interfering with upstream cell signaling and contributing to cancer cell growth inhibition. |
| General description: | Research area: Apoptosis. Azurinis a periplasmic protein and is a homotetramer. |
| Packaging: | 1 mg in glass bottle |
| Physical form: | Lyophilized powder containing ammonium acetate buffer salts. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |