Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat
SIGMA/A3682 - affinity isolated antibody, buffered aqueous solution
Synonym: Goat Anti-Mouse IgG (Fab specific)- HRP
MDL Number: MFCD00162623
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded human appendix sections stained with Monoclonal Anti-Actin, α-Smooth Muscle (Cat. No. A2547) followed by 1:150 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A3682).
Western Blotting Whole extract of Hela cells was separated on SDS-PAGE and probed with Anti-β-Actin antibody, Mouse monoclonal (Cat. No. A1978). The antibody was developed using1:80,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A3682) and chemiluminescent substrate. Lanes 1. 1:80,000 antibody 2. 1:160,000 antibody
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | human, rat |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:40,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150 | |
| western blot: 1:80,000-160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per lane) |
| Application: | Murine IgG levels were quantitated in brain abscess homogenates by ELISA using HRP conjugated goat anti-mouse Fab specific antibody as the detection antibody. |
| Application: | Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in goat has been used in: • enzyme-linked immunosorbent assay (ELISA) • immunohistochemical studies • dot or immunoblotting |
| Application: | Goat anti-mouse IgG (Fab specific)-peroxidase antibody can be used for western blotting applications. The product can also be used for direct ELISA (1:40,000) and immunohistochemistry (at 1:150, using formalin-fixed, paraffin-embedded sections). |
| Application: | HRP conjugated goat anti-mouse IgG Fab specific was used as the secondary antibody for western blot analysis of bacterial samples of s. equi cells to detect fibrinogen-binding proteins. |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG2a has cytotropic properties and IgG2 molecules have the ability to activate the complement system. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mice possess five classes of immunoglobulins- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. |
| General description: | Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Goat anti-mouse IgG (Fab specific)-peroxidase antibody is specific for mouse IgG and the Fab fragment of mouse IgG. The antibody reacts with all mouse IgG subclasses (G1, G2a, G2b and G3) and also with mouse IgA, IgM and IgE. Moreover, the antibody conjugate shows no reactivity with the Fc fragment of mouse IgG or with human and rat IgG. |
| Immunogen: | purified mouse IgG, Fab fragment |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT |
| Preparation Note: | Adsorbed with human IgG and rat serum proteins to reduce background staining with human or rat samples. |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |