Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat
SIGMA/A3687 - affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Alkaline Phosphatase Secondary Antibody; Anti Rabbit Antibody - Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat; Anti Rabbit Sigma; anti rabbit antibody
MDL Number: MFCD00162782
Product Type: Chemical
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous glycerol solution |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | rabbit |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:30,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 | |
| western blot: 1:30,000 |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Dot blot (1 paper) Enzyme-linked immunosorbent assay (1 paper) Immunohistochemistry (1 paper) Western Blotting (10 papers) |
| Application: | Citrullination of antithrombin by PADI4 was analyzed by ELISA using alkaline phosphatase conjugated goat anti-rabbit IgG as the secondary diluted at 1:5000 in 0.05M carbonate/bicarbonate buffer (Ph 9.6). |
| Application: | Goat anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody has been used for western blot and ELISA applications.the antibody can also be used for immunohistochemistry (at 1:50, using formalin-fixed, paraffin-embedded sections). |
| Application: | The level of hypoxyprobe binding proteins present in retinal mouse tissue was analyzed by ELISA using alkaline phosphatase conjugated goat anti-rabbit IgG as the secondary at a dilution of 1:2000 in 5% tween/PBS. Substrate used was 4-nitrophenyl phosphate disodium salt hexahydrate (Sigma). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody binds all rabbit Igs. |
| Immunogen: | purified rabbit IgG as the immunogen |
| Physical form: | Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |