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Acrylamide/Bis-acrylamide, 30% solution

SIGMA/A3699 - BioReagent, suitable for electrophoresis, 37.5:1

Synonym: 30% Acrylamide/Bisacrylamide 37.5:1; Polyacrylamide solution

MDL Number: MFCD00080848
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-A3699-100ML 100 mL
 $92.50
1/EA		 Add To Favorites
45-A3699-5X100ML 100 mL
 $174.00
5/EA		 Add To Favorites

 

feed ratio 37.5:1
form liquid
InChI 1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)
InChI key OCQZXMCGTAWGEQ-UHFFFAOYSA-N
product line BioReagent
Quality Level 100 
SMILES string NC(=O)C=C.C=CC(=O)NCNC(=O)C=C
sterility 0.2 μm filtered
storage temp. 2-8°C
suitability suitable for electrophoresis
technique(s) electrophoresis: suitable
Application: Acrylamide/Bis-acrylamide, 30% solution has been used:
• in the preparation of polyacrylamide gel for photobleaching experiment
• as a constituent in the preparation of gel precursor solution and pore-gradient microgel precursor solution
• in the preparation of polyacrylamide gels during western blot analyses
Application: These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.
General description: Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.
Preparation Note: Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.
Storage Temp. 2-8°C; 2-8°C
UNSPSC 41105319

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