Asparaginase from Escherichia coli
SIGMA/A3809 - lyophilized powder, 100-300 units/mg protein (biuret)
Synonym: L-Asparagine Amidohydrolase
CAS Number: 9015-68-3
EC Number: 232-765-3
MDL Number: MFCD00130565
Product Type: Chemical
Enzymatic Assay Enzymatic assay to measure the inecrease in liberated NH3 by absorbance at 480 nm, through exposure of L-Asparagine to Asparaginase and H2O. Stochiometry L-Asparagine + H2O + Asparaginase → L-Aspartate + NH3
Enzymatic Assay Enzymatic assay to measure the increasement in liberated NH3 by absorbance at 480nm, through exposure of L-Asparagine to Asparaginase and H2O. Stoichiometry: L-Asparagine + H2O + Asparaginase → L-Aspartate + NH3
| composition | Protein, ≥60% |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | 100-300 units/mg protein (biuret) |
| storage temp. | 2-8°C |
| Analysis Note: | Protein determined by biuret. |
| Application: | Asparaginase from Escherichia coli has been used: • to compare the cytotoxic effect of • as a standard in asparaginase assay to quantify asparaginase activities in various eel tissues • to elicit amino acid deprivation |
| Biochem/physiol Actions: | Asparaginase (ASNase) products are usually obtained from Escherichia coli and Erwinia chrysanthemi. These enzymes can block the synthesis of protein in tumor cells. It shows high activity in the G1 phase of the cell cycle. It is capable of causing pancreatitis in leukemia patients. |
| Biochem/physiol Actions: | Asparaginase is used in enzymatic assays and to convert asparagine to aspartic acid. Asparaginase is used to reduce the formation of acrylamide in starchy food products. It is also used as a chemotherapy agent for acute lymphoblastic leukemia . Product A3809 is from Escherichia coli and is provided as a lyophilized powder containing sodium chloride. |
| Biochem/physiol Actions: | Asparaginase may cause cell death in leukemic cells by converting the necessary L-asparagine to aspartic acid and ammonia . Asparaginase is allosterically regulated and is crucial for proper cell functioning . |
| General description: | Asparaginase is a bacterial enzyme and also a chemotherapeutic drug. |
| Packaging: | 100, 1000 units in serum bottle |
| Physical form: | Lyophilized powder containing sodium chloride |
| Preparation Note: | Chromatographically purified |
| Unit Definition: | One unit will liberate 1.0 μmole of ammonia from |
| Symbol |   GHS07,GHS08 |
| Signal word | Warning |
| Hazard statements | H317 - H361 |
| Precautionary statements | P201 - P280 - P302 + P352 - P308 + P313 |
| Hazard Codes | Xn |
| Risk Statements | 63-42/43 |
| Safety Statements | 22-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 100-300 units/mg protein (biuret) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.5.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |