Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat
SIGMA/A3812 - affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat
MDL Number: MFCD00162782
Product Type: Chemical
antibody form | affinity isolated antibody |
antibody product type | secondary antibodies |
biological source | goat |
clone | polyclonal |
conjugate | alkaline phosphatase conjugate |
form | buffered aqueous glycerol solution |
Quality Level | 200 |
shipped in | wet ice |
species reactivity | rabbit |
storage temp. | 2-8°C |
target post-translational modification | unmodified |
technique(s) | direct ELISA: 1:30,000 |
dot blot: 1:30,000 | |
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 | |
western blot: 1:30,000 |
Application: | Citrullination of antithrombin by PADI4 was analyzed by ELISA using alkaline phosphatase conjugated goat anti-rabbit IgG as the secondary diluted at 1:5000 in 0.05M carbonate/bicarbonate buffer (Ph 9.6). |
Application: | Goat anti-rabbit IgG (whole molecule)-alkaline phosphatase antibody can be used for western blot and ELISA |
Application: | It can also be used for immunohistochemistry (1:50) and dot bot (1:30,000) assays. |
Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
General description: | Anti-rabbit IgG (whole molecule) is produced in goat using purified rabbit IgG as the immunogen. Antibody is isolated from anti-rabbit IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins that do not specifically bind to rabbit IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations. Anti-Rabbit IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde. Specificity of the antiserum is determined by immunoelectrophoresis prior to conjugation, versus normal rabbit serum and rabbit IgG. Identity and purity of the antibody is established by immunoelectrophoresis prior to conjugation. Electrophoresis of the product followed by diffusion versus anti-goat IgG and anti-goat whole serum results in single arcs of precipitation. |
General description: | IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. |
General description: | Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It consists of a gamma (γ) heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity (Fab fragments). The third fragment does not possess antigen-binding activity and is known as fragment crystallizable (Fc). |
Immunogen: | Purified rabbit IgG |
Physical form: | Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide |
Preparation Note: | Adsorbed to reduce background staining with human samples. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 2 |
Storage Temp. | 2-8°C |
UNSPSC | 12352203 |