Anti-Actin antibody, Mouse monoclonal
SIGMA/A3853 - clone AC-40, purified from hybridoma cell culture
Synonym: Anti-ACTA
Product Type: Chemical
Western Blotting JOURNAL CITATION: Inhibition of mTOR by rapamycin abolishes cognitive deficits and reduces amyloid-beta levels in a mouse model of Alzheimer′s disease. By: Spilman, P., Podlutskaya, N., et al. in PLoS One, 2010. PubMed ID: 20376313 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0009979), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Inhibition of mTOR by rapamycin abolishes cognitive deficits and reduces amyloid-beta levels in a mouse model of Alzheimer′s disease. By: Spilman, P., Podlutskaya, N., et al. in PLoS One, 2010. PubMed ID: 20376313 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0009979), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Duodenal expression of iron transport molecules in patients with hereditary hemochromatosis or iron deficiency. By: Dostalikova-Cimburova, M., Kratka, K., et al. in J Cell Mol Med, 2012. PubMed ID: 21973163 Image collected and cropped by CiteAb from the following publication, (http://doi.wiley.com/10.1111/j.1582-4934.2011.01458.x), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human appendix sections with 10 μg/mL Monoclonal Anti-Actin Clone: AC-40 (Cat. No. A3853) using biotin/ExtrAvidin®-Peroxidase.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human heart sections with 10 μg/mL Monoclonal Anti-Actin Clone: AC-40 (Cat. No. A3853) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence Rat2 cells were fixed and permeabilized with methanol followed by methanol:aceton. Fixed cells were stained with 2 μg/mL Monoclonal Anti-Actin Clone: AC-40 (Cat. No. A3853). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Monoclonal Anti-Actin Clone: AC-40 (Cat. No. A3853). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. JURKAT 3. COS7 4. NIH-3T3 5. PC-12 6. RAT2 7. CHO 8. MDBK 9. MDCK
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | AC-40, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 10-20 μg/mL using chicken or human fibroblasts by methanol/acetone fixation |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.5 μg/mL using chicken or human fibroblasts |
| Application: | Monoclonal anti-actin antibody was used for western blot analysis of Cos-7 cell lysates to ensure equal protein loading. |
| Application: | Mouse monoclonal anti-actin antibody can be used for western blot assays. Furthermore, the product can also be used for immunocytochemistry (10-20μg/mL, using chicken or human fibroblasts by methanol/acetone fixation), microarray, immunohistochemistry and indirect ELISA. Monoclonal Anti-Actin antibody produced in mouse has been used in the detection of actin in human bronchial epithelial cells using immunohistochemical assays. It has also been used in immunofluorescence detection in cardiomyocytes. |
| Application: | Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody. |
| Biochem/physiol Actions: | Actin and myosin are constituents of many cell types and are involved in cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. The N-terminal region of actin appears to be a major antigenic region, and may be involved in the interaction of actin with other proteins such as myosin. Among the six isoforms of actin, four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. There are three α-actins (skeletal, cardiac, and smooth muscle), one β-actin (β-nonmuscle), and two γ-actins (γ-smooth muscle and γ-nonmuscle). Actin isoforms show >90% overall sequence homology, but only 50–60% homology in their NH2-terminal residues. The actin in cells of various species and tissues are very similar in their immunological and physical properties characterized by electrophoresis and amino acid sequence analysis. They mediate cytoskeletal remodeling during T cell activation. Mutation in the γ-actin and β-actin gene is associated with a developmental disorder called Baraitser-Winter syndrome. Mutation in the γ-actin leads to hearing loss. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three α actins (skeletal, cardiac, smooth), one β actin (β-non-muscle) and two γ actins (γ smooth muscle and γ non-muscle). The human γ-actin gene is mapped to human chromosome 17. The antibody recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms. It specifically labels actin in a wide variety of tissues and species. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Zinc-formalin, B5, ethanol, methacarn, Brunnel′s or Bouin′s solutions may also be used as fixatives. Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. A synthetic actin C-terminal peptide, attached to Multiple Antigen Peptide (MAP) backbone was used as the immunogen. The isotype is determined using Mouse Monoclonal Antibody Isotyping Reagents, Catalog No. ISO2 |
| General description: | The antibody recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms. It specifically labels actin in a wide variety of tissues and species. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Zinc-formalin, B5, ethanol, methacarn, Brunnel′s or Bouin′s solutions may also be used as fixatives. |
| Immunogen: | synthetic actin C-terminal peptide, attached to a Multiple Antigen Peptide (MAP) backbone. |
| Other Notes: | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/acti. |
| Packaging: | 200 μL in glass insert |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Cross-reaction of monoclonal Anti-Actin antibody has been observed with human, bovine, sheep, goat, pig, rabbit, dog, mouse, rat, guinea pig, hamster, chicken, carp, viper, frog and snail. The antibody may be used in staining of methanol-fixed, frozen sections. |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |