Aprotinin from bovine lung
SIGMA/A3886
Synonym: BPTI; Bovine pancreatic trypsin inhibitor; Trasylol; Trypsin inhibitor (basic)
CAS Number: 9087-70-1
Empirical Formula (Hill Notation): C284H432N84O79S7
Molecular Weight: 6511.44
EC Number: 232-994-9
MDL Number: MFCD00130541
Linear Formula: C284H432N84O79S7
Product Type: Chemical
biological source | bovine lung |
form | powder |
InChI key | ZPNFWUPYTFPOJU-UHFFFAOYSA |
mol wt | ~6,500 |
packaging | vial of 15 mg |
Quality Level | 200 |
solubility | glycerol: 3 mg/mL, clear, colorless (equilibration buffer containing 5% glycerol) |
storage temp. | −20°C |
UniProt accession no. | P00974 |
Application: | Aprotinin is largely used as an inhibitor of trypsin. |
Application: | Gel filtration molecular weight marker |
Biochem/physiol Actions: | Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges. |
Unit Definition: | One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-argini |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
UNSPSC | 12352200 |