Anti-β-Amyloid antibody, Mouse monoclonal
SIGMA/A3981 - clone BAM-10, purified from hybridoma cell culture
Synonym: Anti-A-BETA; Anti-Amyloid β Precursor Protein; Clone BAM91
MDL Number: MFCD00803867
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Formalin fixed, paraffin-embedded, methacarn-treated sections of Human Alzheimer’s disease (AD) brain were stained with 1:100 Anti-β-Amyloid Protein (1-40) antibody produced in Rabbit (Cat. No. A8326) (A) and developed using 15 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886), and 2.5 μg/mL Anti-β-Amyloid antibody, Mouse monoclonal, Clone: BAM-10 (Cat. No. A3981) (B). The antibody was developed using using 15 μg/mL Anti-Mouse IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B7151) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886). Results Two Anti-β-Amyloid antibodies,A8326 (A) and A3981 (B) target different regions of β-Amyloid show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human Alzheimer′s disease (AD) brain sections with 3 μg/mL Monoclonal Anti-β-Amyloid Antibody Clone: BAM-10 (Cat. No. A3981) using biotin/ExtrAvidin®-Peroxidase.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | BAM-10, monoclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| isotype | IgG1 |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5-5 μg/mL using formic-acid treated tissue sections of human Alzheimer disease brain tissue |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | P05067  |
| Application: | Monoclonal Anti-β-Amyloid antibody has been used in immunoprecipitation analysis, microarray-based immunoassay and microarray. |
| Application: | Mouse monoclonal anti-ABETA was used to treat old WT PDAPP mice with amyloid accumulation and learning deficits in an attempt to improve learning and decrease accumulation, however no response was observed. |
| Application: | The antibody is useful in immunohistochemistry, immunoblotting, ELISA, and competitive ELISA. Also, this antibody has been used to neutralize Aβ assemblies in brains of transgenic mice expressing a mutant form of amyloid precursor protein, and for in vivo deep tissue imaging using near-IR optical spectrum. |
| Biochem/physiol Actions: | β-amyloid gene acts as the substrate of insulin-degrading enzyme (IDE). It plays a major role in the pathogenesis of Alzheimer’s disease (AD) and type 2 diabetes mellitus (DM2). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | β-amyloid gene is located on human chromosome 21q21. |
| General description: | The β-amyloid precursor protein (APP) is cleaved sequentially by the proteolytic enzymes β-and γ-secretase to produce β-amyloid (Aβ) peptides with the Aβ1-42 and the Aβ1-40 forms being the most prevalent. Secreted Aβ peptides are degraded either via a re-uptake mechanism followed by endosomal degradation, or by an extracellular insulin degrading enzyme. Extracellular accumulation of Aβ leads to the formation of aggregates, fibrils and eventually amyloid deposits called neuritic plaques, which is the hallmark of Alzheimer′s disease (AD). |
| General description: | The antibody reacts specifically with β-amyloid protein. The epitope recognized by the antibody resides within amino acids 1-12 of the β-amyloid protein. It specifically stains amyloid plaques within the cortex and amyloid deposits in blood vessels using formic acid-treated, formalin-fixed, paraffin-embedded, and Methacarn-fixed sections of human Alzheimer′s disease (AD) brain tissue. |
| Immunogen: | Synthetic β-amyloid peptide, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |