Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit
SIGMA/A4062 - affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Rabbit Anti-Goat IgG (whole molecule)–AP
MDL Number: MFCD00162335
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human tonsil tissue sections were stained with 1:50 Anti-Human IgG (whole molecule) antibody produced in Goat (Cat. No. I1011) followed by 1:100 Anti-Goat IgG (whole molecule)-Alkaline Phosphatase antibody produced in Rabbit (Cat. No. A4062).
Western Blotting Goat IgG were separated on SDS-PAGE, probed and developed using Anti-Goat IgG (whole molecule)-Alkaline Phosphatase antibody produced in Rabbit (Cat. No. A4062). Lanes 1. 1:30,000 antibody 2. 1:60,000 antibody
ELISA Anti-Goat IgG (whole molecule)-Alkaline Phosphatase antibody produced in Rabbit (Cat. No. A4062), was serially diluted to detect Goat IgG by direct ELISA. Reactivity is observed with Goat IgG, but not with Human IgG and NHS.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous glycerol solution |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | goat |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:30,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 | |
| western blot: 1:30,000 |
| Application: | Alkaline phosphatase conjugated rabbit anti goat IgG was used as the secondary for ELISA assays to detect the binding of bacterial proteins to C components. The antibody was used at 1:5000 dilutions and detected using P-nitrophenyl phosphate at 1mg/ml (Sigma) as the substrate. |
| Application: | Anti-Goat IgG (whole molecule)-alkaline phosphatase antibody can be used in western blotting and immunoblotting (diluted 1: 20,000) . |
| Biochem/physiol Actions: | IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus, they play a crucial role in facilitating cytological immune responses. Anti-goat IgG (whole molecule)-alkaline phosphatase antibody (diluted 1: 5000) can be used for anti-OVA IgG detection. Rabbit anti- goat IgG (whole molecule)-alkaline phosphatase antibody reacts specifically with all goat immunoglobulins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulins (Igs) belongs immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobin have two heavy (H) and two light (L), held together by disulphide linkages. Each light comprises of one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. |
| Immunogen: | purified goat IgG |
| Other Notes: | Antibody adsorbed with human serum proteins. |
| Physical form: | Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 10 mM glycine, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide |
| Preparation Note: | Adsorbed to reduce background staining with human samples. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |