Anti-ATF-2 (SS-16) antibody produced in rabbit
SIGMA/A4086 - affinity isolated antibody, buffered aqueous solution
MDL Number: MFCD01092263
Product Type: Chemical
Western Blotting JOURNAL CITATION: Cdk1-mediated phosphorylation of human ATF7 at Thr-51 and Thr-53 promotes cell-cycle progression into M phase. By: Hasegawa, H., Ishibashi, K., et al. in PLoS One, 2014. PubMed ID: 25545367 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0116048), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting Whole extract of Jurkat cells were separated on SDS-PAGE and probed with Anti-ATF-2 (SS-16) antibody produced in Rabbit (Cat. No. A4086). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:250 antibody 2. 1:500 antibody 3. 1:500 + inhibitor 4. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 65 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5 μg using lysates of human K562 cells |
| microarray: suitable | |
| western blot (chemiluminescent): 1:250 using whole cell extract of human K562 or Jurkat cells | |
| UniProt accession no. | P15336  |
| Application: | Rabbit anti-ATF-2 can be used for immunoprecipitation (5μg, using lysates of human K562 cells), microarray, and chemiluminescent western blot (1:250, using whole cell extract of human K562 or Jurkat cells). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | ATF2 (Activating Transcription Factor 2, CRE-BP,HB-16, CREB2, TREB-7) belongs to the ATF/CREB family of basic region leucine zipper DNAbinding proteins. ATF2 regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Rabbit anti-ATF-2 (SS-16) antibody recognizes human ATF2 by immunoblotting (65kDa) and immunoprecipitation. Staining of ATF2 by immunoblotting is inhibited by the immunizing peptide. |
| Immunogen: | synthetic peptide corresponding to amino acids 490-505 of human ATF-2. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
| WGK Germany | - |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |