Anti-phospho-ATF-2 (pThr69,71) antibody, Mouse monoclonal
SIGMA/A4095 - clone ATF-22P, purified from hybridoma cell culture
Synonym: Monoclonal Anti-phospho-ATF-2 (pThr69,71)
MDL Number: MFCD03096614
Product Type: Chemical
Western Blotting Whole extract of Jurkat cells were separated on SDS-PAGE and probed with Anti-phospho-ATF-2 (pThr69,71) antibody, Mouse monoclonal, Clone: ATF-22P (Cat. No. A4095). The antibody was developed using 1:1,000 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A2179). Lanes 1. 1 μg/mL antibody 2. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | ATF-22P, monoclonal |
| concentration | 0.5-1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 60-70 kDa (triplet) |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | frog, mouse, rat, chicken, human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pThr69/pThr71) |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: suitable | |
| UniProt accession no. | P15336  |
| Application: | Mouse monoclonal anti-phospho-ATF-2 (pThr69,71) antibody can be used for immunocytochemistry, indirect ELISA, microarray, and western blot. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | ATF2 (Activating Transcription Factor 2) is a member of the leucine zipper family of transcription factors. It binds to CRE domains and stimulates CRE-dependent transcription. |
| General description: | Mouse monoclonal anti-phospho-ATF-2 (pThr69,71) reacts specifically with ATF2 phosphorylated at both threonine 69 and 71, and does not detect the monophosphorylated and the non-phosphorylated ATF2 molecule. The epitope recognized by the antibody resides in the N-terminal region of ATF2 (aa 66-79, pT69,71). This epitope is identical in ATF2 of human, rat, mouse, chicken and frog. The antibody cross-reacts with human, rat and mouse ATF2. |
| Immunogen: | synthetic peptide corresponding to the N-terminal amino acids 66-79 (pT69,71) of human ATF2. This sequence is identical in ATF2 from human, rat, mouse, chicken, and frog. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| Preparation Note: | Purified from culture supernatant of hybridoma cells grown in a bioreactor. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |