Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit
SIGMA/A4174 - affinity isolated antibody, buffered aqueous solution
Synonym: Rabbit Anti-Goat IgG (whole molecule)–HRP
MDL Number: MFCD00162340
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Rabbit spleen were stained with 1:500 Anti-Rabbit IgG (whole molecule) antibody produced in Goat (Cat. No. R0881), followed by 1:500 Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A4174).
Immunohistochemistry Formalin fixed, paraffin-embedded Rabbit spleen were stained with 1:500 Anti-Rabbit IgG (whole molecule) antibody produced in Goat (Cat. No. R0881), followed by 1:500 Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A4174).
ELISA Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A4174) was serially diluted to detect Goat IgG by direct ELISA. Reactivity is observed only with Goat IgG but not with HuIgG and NHS.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | human |
| species reactivity | goat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:10,000 |
| Application: | Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in rabbit is suitable for use in immunoblot and immunohistochemistry. The product can also be used for direct ELISA (1:10,000). |
| Application: | The presence of pleiotrophin in HUVEC cell culture medium was analyzed by western blot using HRP-conjugated rabbit anti-goat IgGas the secondary at a dilution of 1:7500 in TBST. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus they play a crucial role in facilitating cytological immune responses. Anti-goat IgG (whole molecule)–peroxidase antibody can be used as a secondary antibody in ovarian tissue microarray. Rabbit anti-goat IgG (whole molecule)-peroxidase antibody reacts specifically with all goat IgG but shows no reactivity with human serum proteins. |
| Immunogen: | Purified goat IgG. |
| Other Notes: | Antibody adsorbed with human serum proteins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT. |
| Preparation Note: | Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |