Monoclonal Anti-γ-Adaptin antibody produced in mouse
SIGMA/A4200 - clone 100/3, ascites fluid
Synonym: Anti-AP-1
MDL Number: MFCD00212879
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: CD2v Interacts with Adaptor Protein AP-1 during African Swine Fever Infection. By: Pérez-Núñez, D., García-Urdiales, E., et al. in PLoS One, 2015. PubMed ID: 25915900 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0123714), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry-immunoflourescence JOURNAL CITATION: CD2v Interacts with Adaptor Protein AP-1 during African Swine Fever Infection. By: Pérez-Núñez, D., García-Urdiales, E., et al. in PLoS One, 2015. PubMed ID: 25915900 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0123714), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry-immunoflourescence JOURNAL CITATION: CD2v Interacts with Adaptor Protein AP-1 during African Swine Fever Infection. By: Pérez-Núñez, D., García-Urdiales, E., et al. in PLoS One, 2015. PubMed ID: 25915900 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0123714), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence
Immunofluorescent staining of 3T3 fibroblasts with Monoclonal Anti-γ-Adaptin (AP-1, Clone 100/3) (Cat. No. A4200).
From E. Ungewickell, Washington Univ. Sch. of Medicine, St. Louis, MO.
Immunofluorescent staining of 3T3 fibroblasts with Monoclonal Anti-γ-Adaptin (AP-1, Clone 100/3) (Cat. No. A4200).
From E. Ungewickell, Washington Univ. Sch. of Medicine, St. Louis, MO.
Immunoblotting Bovine brain extract was separated on SDS-PAGE and probed with Monoclonal AB to Gamma Adaptin (AP-1)(MO) CL: 100/3 (PREPK) (Cat. No. A4200). The antibody was developed with Alkaline Phosphatase conjugate (Cat. No. A1293) and a NBT/BCIP substrate. Antibody dilution 1/200.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 100/3, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2b |
| mol wt | antigen 104 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | mouse, rat |
| species reactivity | bovine, human, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | electron microscopy: suitable |
| immunoprecipitation (IP): suitable | |
| western blot: 1:100 using bovine brain extract | |
| UniProt accession no. | O43747  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immuno-electron microscopy (1 paper) Immunofluorescence (1 paper) |
| Application: | Immunofluorescence of Hela cells was performed using monoclonal anti-AP1 (clone 100/3) as the primary antibody. |
| Application: | In immunofluorescence using MDBK cells, human heart fibroblasts, and African green monkey kidney cells (Vero), the binding of antibody appears to be largely confined to the trans-Golgi network. The antibody has been used for studies on the effects of Brefeldin A, a substance causing rapid redistribution of coat proteins associated with the clathrin-coated vesicles that bud from the trans-Golgi network. The antibody is also useful for the immunoaffinity purification of the Golgi adaptor complex AP-1. |
| Application: | Mouse monoclonal anti-gamma-adaptin antibody can be used for western blot (1:100, using bovine brain extract), immunoprecipitation and electron microscopy applications. |
| Biochem/physiol Actions: | Mouse monoclonal clone 100/3 anti-g-Adaptin antibody reacts in immunoblotting with the 104 kDa polypeptide of the Golgi adaptor complex AP-1. It reacts with polypeptides of ~100 kDa in bovine liver, human heart fibroblasts, and Madin-Darby bovine kidney cultured cells (MDBK), but not with any components in the 110-115 kDa range from these sources or from neuroblastoma or astrocytes. This suggests that the 110 and 115 kDa polypeptides may be specific variants occurring only in some cell types of brain. The antibody does not recognize the γ-subunit in rodents (rat and mouse). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Clathrin-coated vesicle populations contain the adaptor complexes AP-1 and AP-2, also known as HA-I (HA1) adaptor and HA-II (HA2) adaptor or Assemble Protein 1 (AP1) and Assemble Protein 2 (AP2), respectively. Clathrin-coated membranes at or originating from the plasma membrane contain the AP-2 adaptor, while the AP-1 adaptor appears to be largely restricted to clathrin-coated membranes of the trans-Golgi network. Both the Golgi-associated AP-1 adaptor and the plasma-membrane associated AP-2 adaptor are heterotetrameric protein complexes. AP-1 adaptor consists of four subunits termed b1 (formerly b′, 110 kDa), g (100 kDa), m1 (47 kDa), and s1 (20 kDa). b1 and g subunits (b1 and g adaptins) from neuronal sources behave in standard SDS-PAGE like 115 kDa and 104 kDa polypeptides, respectively. |
| Immunogen: | AP-1 adaptor from bovine brain. |
| Physical form: | The product is provided as ascites fluid with 0.1% sodium azide as a preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |