α-Amylase from porcine pancreas
SIGMA/A4268 - Type I-A, PMSF treated, saline suspension, 700-1400 units/mg protein (E1%/280 )
Synonym: β-N-acetylglucosaminidase porcine placenta; PPA; al1,4 glucan-
MDL Number: MFCD00081319
Product Type: Chemical
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
| application(s) | diagnostic assay manufacturing |
| biological source | Porcine pancreas |
| form | saline suspension |
| greener alternative category |  , Enabling |
| greener alternative product characteristics | Waste Prevention Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| mol wt | 51-54 kDa |
| Quality Level | 300 |
| specific activity | 700-1400 units/mg protein (E |
| storage temp. | 2-8°C |
| suitability | suitable for hydrolysis, synthesis of oligosaccharides and polysaccharides, and sugar modification |
| sustainability | Greener Alternative Product |
| technique(s) | activity assay: suitable |
| type | Type I-A |
| Application: | α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. α-Amylase, from Sigma, has been used in various plant studies, such as metabolism studies in Arabidopsis . |
| Biochem/physiol Actions: | α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. α -Amylase, from porcine pancreas, is a glycoprotein that consists of a single polypeptide chain of approximately 475 residues containing 2 SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. |
| General description: | Molecular mass: 51-54 kDa. α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. Chloride ions are necessary for activity and stability. The pH range for activity is 5.5 to 8.0, with the pH optimum at 7. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles . |
| Other Notes: | One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C. |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Physical form: | Suspension in 2.9 M NaCl solution containing 3 mM CaCl2. |
| Preparation Note: | 2× crystallized |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P342 + P311 |
| Hazard Codes | Xn |
| Risk Statements | 42 |
| Safety Statements | 22-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 700-1400 units/mg protein (E |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |