Anti-Human IgM (μ-chain specific) F(ab′)2 fragment−Peroxidase antibody produced in goat
SIGMA/A4290 - affinity isolated antibody, buffered aqueous solution
MDL Number: MFCD00162464
Product Type: Chemical
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:10,000 |
| Application: | Goat polyclonal anti-Human IgM (μ-chain specific) F(ab′)2 fragment−Peroxidase antibody may be used to detect and quantitate the level of IgM in human serum and biological fluids via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques. ELISA assays on EBV +BCLs established from EBV+ human B cells were performed using HRP conjugated goat anti-human IgM mu chain specific as the secondary. Secondary antibody was incubated for 2 hrs at room temperature. |
| Application: | Peroxidise-conjugated goat F(ab′)2 fragment anti-human IgM (μ-chain specific) has been used as a secondary antibody to detect human IgMs to hantanvirus antigens using ELISA. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Goat polyclonal anti-Human IgM (μ-chain specific) F(ab′)2 fragment−Peroxidase antibody is specific for human IgM when tested against purified human IgA, IgG, IgM, Bence Jones Kappa and Bence Jones Lambda myeloma proteins. |
| General description: | Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM antibodies are responsible for agglutination of red blood cells in mis-matched blood transfusions. The level of IgM may vary with the status of disease or infection. Horseradish Peroxidase (HRP) is an enzyme that catalyzes the conversion of chromogenic substrates such as o-phenylenediamine (OPD), 4-chloro-1-naphthol 3,3’,5,5’-tetramethylbenz |
| Immunogen: | purified human IgM |
| Legal Information: | Ampliflu is a trademark of Sigma-Aldrich Co. LLC |
| Packaging: | 1 mL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative. |
| Preparation Note: | Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978). |
| Symbol |   GHS07,GHS09 |
| Signal word | Warning |
| Hazard statements | H315 - H317 - H319 - H411 |
| Precautionary statements | P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |