Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit
SIGMA/A4312 - affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Rabbit Anti-Mouse IgG (whole molecule)−AP
MDL Number: MFCD00162640
Product Type: Chemical
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with 2 μg/mL Anti-β-Actin antibody produced in Mouse. The antibody was developed using Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody produced in Rabbit (Cat. No. A4312). Lanes 1. 1:30,000 antibody 2. 1:60,000 antibody 3. Negative Control
Immunohistochemistry Formalin-fixed, paraffin-embedded section of human appendix stained with Monoclonal Anti-Actin, α-Smooth Muscle (Cat. No. A2547) followed by 1:50 Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody produced in rabbit (Cat. No. A4312)
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous glycerol solution |
| Quality Level | 300  |
| shipped in | wet ice |
| species reactivity | mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:30,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 | |
| western blot: 1:30,000 using using β-Actin in total cell extract of HeLa cells (5-10 μg/well) |
| Application: | Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in rabbit has been used in immunocytochemical localization of Aβ peptides and western blot analysis. |
| Application: | Anti-mouse IgG (whole molecule)-alkaline phosphatase antibody can be used in immunocytochemistry. |
| Application: | Western blot analysis of lysates from the placental cell line 3ASubE-P-3 and U037 cells were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:2000 to recognize the mouse mom Il-8RB. Secondary was developed using bromochloroindoyl phosphate (Sigma) pH. 9.5 for 10-15 minutes.Western blot analysis of coimmunoprecipitates from human cell or rat cell vaginal extracts were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:15000 dilution. |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG antibodies are the smallest antibody isotype found in blood and extracellular fluid, capable of fighting bacterial and viral infection. |
| General description: | Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. |
| Immunogen: | Purified mouse IgG. |
| Physical form: | Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |