Albumin from human serum
SIGMA/A4327 - lyophilized powder, essentially protease free, ≥96% (agarose gel electrophoresis)
Synonym: HSA
CAS Number: 70024-90-7
EC Number: 274-272-6
MDL Number: MFCD00081418
Product Type: Chemical
Human serum albumin is a 66 kDa predominantly monomeric protein with one free sulfhydryl group and 17 intrachain disulfide bonds.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A4327-500MG
| assay | ≥96% (agarose gel electrophoresis) |
| biological source | human |
| foreign activity | Alkaline phosphatase ≤0.001 units/mg protein |
| Peroxidase ≤0.001 units/mg protein | |
| Protease ≤0.0001 units/mg protein | |
| form | lyophilized powder |
| impurities | HIV I and HIVII, HCV and HBsAg, tested negative |
| InChI | 1S/C3F8/c4-1(5,2(6,7)8)3( |
| InChI key | QYSGYZVSCZSLHT-UHFFFAOYSA |
| mol wt | monomer calculated mol wt 66478 Da |
| Quality Level | 200  |
| solubility | H2O: soluble 50 mg/mL |
| storage temp. | 2-8°C |
| technique(s) | tissue culture: suitable |
| UniProt accession no. | P02768  |
| Application: | Albumin was used to test its effect on the in vitro bactericidal activity of cefditoren against penicillin-resistant Streptococcus pneumonia. It has been clinically used in serious and often life-threatening conditions, such as shock and blood loss due to trauma, burns, and surgery. It was used to test the effect of non-enzymatic glycation on the unfolding of human serum albumin. It was also used to test the two distinct pathways used by intestinal epithelial cells for HLA class II antigen processing. |
| Biochem/physiol Actions: | Serum albumin functions as a carrier protein for steroids, fatty acids, and thyroid hormones, and is vital in regulating the colloidal osmotic pressures of blood. Albumin is also seen to bind to exogenous substances, particularly drugs (e.g., ibuprofen, warfarin), and strongly influence their pharmacokinetics. Oxidative stress leading to changes in the redox state of albumin has widely varied effects on its physiological function. |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| Features and Benefits: | • Low protease activity / essentially protease-free (≤ 0.0001 units/mg protein) • Low alkaline phosphatase activity (≤ 0.001 units/mg protein) • Low peroxidase activity (≤ 0.001 units/mg protein) |
| General description: | Albumin is the most copious protein in blood plasma. Produced in the liver, serum albumin serves as a carrier protein for biomolecules like as fatty acids, amino acids, and steroids. This human serum albumin (HSA) product has been specially tested and evaluated to be low in the following properties: • Essentially protease-free / low protease content • Low alkaline phosphatase content • Low peroxidase content Serum albumin is used in various applications related to molecular biology, notably as an inert protein stabilizer for stock solutions of enzymes and proteins of interest. In such applications, it is thus important for the HSA to be protease free, to maintain the integrity of the protein or enzyme of interest. Protease-free HSA is especially useful for applications like: • Enzyme assays • Protein-based assays • Protease-sensitive techniques such as: (a) Enzyme immunoassay (EIA) (b) Nucleic acid hybridization (c) Radioimmunoassay (RIA) |
| Other Notes: | View more information on human serum albumin . |
| Packaging: | 1 g in glass bottle |
| Packaging: | 500 mg in glass bottle |
| Preparation Note: | Prepared from A1653 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥96% (agarose gel electrophoresis) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |