Activin A active human
SIGMA/A4362 - Animal-component free, recombinant, expressed in Nicotiana, >97% (SDS-PAGE)
Synonym: Erythroid differentiation protein; Inhibin beta A chain; Activin beta-A chain
Product Type: Chemical
Immunoblotting Activin A active humanCat. No. A4362: SDS-PAGE analysis of recombinant Activin A. Samples were loaded in 15% SDS polyacrylamide gel and stained with Coomassie blue. Lanes 1. Molecular weight marker (kDa) 2. 0.2 μg recombinant Activin A
Immunoblotting Activin A active humanCat. No. A4362: Western Blot analysis of recombinant Activin A. Lanes 1. Molecular weight marker (kDa) 2. 0.2 μg Activin A
| assay | >97% (SDS-PAGE) |
| biological source | human |
| form | lyophilized |
| impurities | Endotoxin, tested (LAL test, < 0.04 EU/ ug protein) |
| mol wt | 27.4 kDa (disulfide-linked homodimers of two βA chains, each containing 116 amino residues) |
| packaging | pkg of 5 μg |
| potency | ≤5 ng/mL ED50 |
| Quality Level | 100  |
| recombinant | expressed in Nicotiana |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| UniProt accession no. | P08476  |
| Amino Acid Sequence: | HisHisHisHisHisHisGlyLeuG |
| Analysis Note: | The biological activity of Activin A is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation. ED50: ≤ 5 ng/mL |
| General description: | Activins are homodimers or heterodimers of the various β subunit isoforms, belonging to the TGFβ family. Mature Activin A has two 116 amino acids residues βA subunits (βA-βA). Activin exhibits a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins plays a key role in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Cells known to express Activin A include fibroblasts, endothelial cells, hepatocytes, vascular smooth muscle cells, macrophages, keratinocytes, osteoclasts, bone marrow monocytes, prostatic epithelium, neurons, chondrocytes, osteoblasts, Leydig cells, Sertoli cells, and ovarian granulosa cells. As with other members of the super-family, Activins interact with two types of cell surface trans-membrane receptors (Types I and II) which have intrinsic serine/threonine kinase activities in their cytoplasmic domains, Activin type 1 receptors, ACVR1, ACVR1B, ACVR1C and Activin type 2 receptors, ACVR2A, ACVR2B. The biological activity of Activin A can be neutralized by inhibins and by the diffusible TGF-B antagonist, Follistatin. |
| Other Notes: | Extinction Coefficient: E0.1% = 1.27 (A 280 nm) p.I: 7.27 |
| Physical form: | Lyophilized from a Tris HCl 0.05 M buffer at pH 7.4 |
| Reconstitution: | Lyophilized protein should be reconstituted in water to a concentration of 50 ng /μl. Due to the protein nature, dimmers and multimers may be observed. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |