Monoclonal Anti-β1 and β2-Adaptins antibody produced in mouse
SIGMA/A4450 - clone 100/1, ascites fluid
Synonym: Anti-AP-1 and AP-2
MDL Number: MFCD00212883
Product Type: Chemical
Immunofluorescence
Immunofluorescent staining of 3T3 fibroblast with Monoclonal Anti-β1 and-β2 Adaptins (AP-1 & AP-2, Clone no. 100/1) (Cat. No. A4450).
From E. Ungewickell, Washington Univ. Sch. of Medicine, St. Louis, MO.
Immunofluorescent staining of 3T3 fibroblast with Monoclonal Anti-β1 and-β2 Adaptins (AP-1 & AP-2, Clone no. 100/1) (Cat. No. A4450).
From E. Ungewickell, Washington Univ. Sch. of Medicine, St. Louis, MO.
Western Blotting Whole extract of newborn Rat brain were separated on SDS-PAGE and probed with Monoclonal Anti-β1 and β2-Adaptins antibody produced in Mouse, Clone: 100/1 (Cat. No. A4450). The antibody was developed using 1:1,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1:200 antibody 2. 1:400 antibody 3. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 100/1, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, bovine, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: suitable |
| microarray: suitable | |
| western blot: 1:200 using rat brain extract | |
| UniProt accession no. | P63010  |
| Q10567 |
| Application: | Immunofluorescence of MDCK cells fixed in methanol was performed using anti-AP1 at a dilution of 1:250 and also western blot at a dilution of 1:1000. |
| Application: | Monoclonal Anti-β1 and β2-Adaptins antibody produced in mouse has been used in immunoblotting and immunohistology. |
| Application: | Mouse monoclonal anti-β1 and β2-adaptins can be used for western blot (1:200) assays using rat brain extract. The antibody is also suitable for use in indirect immunofluorescence and microarray analyses. Furthermore, monoclonal anti-β1/β2-adaptins have been used for double staining methods to detect clathrin-coated regions of the plasma membrane in rat brain cells. |
| Biochem/physiol Actions: | β1and β2 adaptins are highly homologous which suggests that the β -type adaptor chains may mediate the interaction of their adaptors with the highly conserved clathrin molecule. Monoclonal antibodies reacting specifically against adaptor proteins are useful tools for studies on the intracellular distribution and structural relationship of adaptor complexes. |
| Biochem/physiol Actions: | Clathrin-coated vesicles facilitate the selective internalization of membrane receptors for lysosomal enzymes from the trans-Golgi network to a pre-lysosomal compartment. Besides clathrin, coated vesicle populations contain the adaptor complexes AP-1 and AP-2, also known as HA-I (HA1) adaptor and HA-II (HA2) adaptor or Assemble Protein 1 (AP1) and Assemble Protein 2 (AP2), respectively. These adaptor proteins mediate the interaction between clathrin and membrane components. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti- β1 and β2-Adaptins (AP-1 & AP-2)(mouse IgG1 isotype) is derived from the 100/1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Adaptins AP-1 and AP-2 are mapped to human chromosome 22q12.2 and 17q12, respectively. AP-1 adaptor consists of four subunits. It includes two large adaptins (one each of γ/α/δ/ε and β1−4 , one medium adaptin (μ1−4), and one small adaptin (ς1−4. Similarly, the AP-2 is made up of two 100-110 kDa polypeptides, termed α and β2 (formerly β) subunits, F2 , and s2 subunit. α and β2 subunits (α and β 2). |
| Immunogen: | AP-2 adaptor protein from bovine brain. |
| Physical form: | The antibody is provided as ascites fluid with 0.1% sodium azide as a preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |