Anti-ARTS antibody, Mouse monoclonal
SIGMA/A4471 - clone ARTS51, purified from hybridoma cell culture
Synonym: Anti-
MDL Number: MFCD03096625
Product Type: Chemical
Immunofluorescence Cos7 cells transfected with ARTS expression vector were stained with Anti-ARTS antibody, Mouse monoclonal, Clone: ARTS51 (Cat. No. A4471). The antibody was developed using Goat Anti-Mouse IgG,FITC conjugate. Cells were counterstained with Mitotracker (red) to stain mitochondria. (The picture is courtesy of Prof. Sarit Larisch, University of Haifa, Israel.)
Immunoblotting Extract of HeLa cells and stable ARTS knockdown (ARTS KD) HeLa cell line were separated on SDS-PAGE and probed with Monoclonal Anti-ARTS, Clone: ARTS51 (Cat. No. A4471) and GAPDH as loading control. (The picture is courtesy of Prof. Sarit Larisch, University of Haifa, Israel.)
Immunoblotting Extract of 293T cells transfected with ARTS plasmid was separated on SDS-PAGE and probed with Monoclonal PA to ARTS (MO) CL:ART S51 (Cat. No. A4471). The antibody was developed with Alkaline Phosphatase conjugate (Cat. No. A2179) and a NBT/BCIP substrate. Antibody dilution 1/4,000.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | ARTS51, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | antigen 32 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.5-1 μg/mL using a whole extract of transfected 293T (human embryonal kidney) cells expressing ARTS |
| Application: | Immunoprecipitation of COS-7 cell lysates was performed using 10 μl of monoclonal mouse anti-ARTS antibody. Antibody was allowed to bind for 4 hours on ice before precipitated using G-sepharose beads. |
| Application: | Monoclonal anti-ARTS antibody has been used for western blot assays. The antibody has also been used for immunostaining applications. Furthermore, the product is suitable for use in indirect ELISA microarray and immunocytochemistry assays. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | 1,2 ARTS was later shown to be a key apoptotic protein mediating apoptosis initiated by other inducers. |
| General description: | Anti-ARTS antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the ARTS51 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide corresponding to C-terminus of human ARTS protein, conjugated to KLH. ARTS (Apoptosis Related protein in the TGF-β signaling pathway), is a novel protein involved in the apoptotic pathways of transforming growth factor-β (TGF-β). The protein is derived from the human septin H5/PNUTL2/CDCrel-2a/2b gene. ARTS consists a phosphate-binding site (P-loop) death domain, which is conserved in all septins and in many different classes of ATP/GTPases. It is localized to mitochondria and translocates to the nucleus, coincident with apoptotic signals induced by TGF-β. |
| General description: | Apoptosis-related protein in TGF-β signaling pathway (ARTS) is a novel mitochondrial protein that mediates apoptosis induced by a variety of pro-apoptotic stimuli. Originally found to be essential for apoptosis induced by transforming growth factor-β (TGF-β) ARTS was later shown to be a key apoptotic protein mediating apoptosis initiated by other inducers. Mouse monoclonal anti-ARTS antibody recognizes an epitope within amino acids 247-266 of human ARTS molecule. |
| Immunogen: | synthetic peptide corresponding to C-terminal region (amino acids 247-266) of human ARTS protein, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | This monoclonal anti-ARTS antibody was used to detect ARTS (Sept4_i2) protein in whole lysates of HeLa cells. HeLa ARTS knockdown (ARTS KD) stable cell line was established by using short hairpin RNAs (shRNAs) which abrogated specifically the endogenous levels of ARTS (Sept4_i2, ~30Kd) This monoclonal Ab is the only currently commercially available antibody directed against the unique C-terminus of ARTS, that can distinguish betweenARTS ( the pro-apoptotic, tumor suppressor, specific XIAP- antagonist) and Sept4_i1 (H5,PNUTL), which is the other product of the SEPT4 gene. |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |