Anti-Annexin VII antibody, Mouse monoclonal
SIGMA/A4475 - clone 203-217/6, purified from hybridoma cell culture
Synonym: Synexin
MDL Number: MFCD03792412
Product Type: Chemical
Immunoblotting Whole extract of A-9 cells was separated on SDS-PAGE, blotted with 0.1 μg/mL Monoclonal Anti-Annexin VII Clone: 203-217/6 (Cat. No. A4475) and developed using Goat Anti-Mouse IgG-ALP (Cat. No. A2179) and a NBT/BCIP (Cat. No. B5655) substrate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 203-217/6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 47-51 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, canine, rat, chicken, bovine, human, rabbit |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable using methanol or 1% paraformaldehyde/0.1% glutaraldehyde |
| immunohistochemistry (frozen sections): suitable | |
| microarray: suitable | |
| western blot: 0.5-1 μg/mL using whole cell extract of culture mouse connective tissue (A9) cells. | |
| UniProt accession no. | P20073  |
| Application: | Anti-Annexin VII antibody, Mouse monoclonal has been used in: • western blotting • immunocytochemistry • immunohistochemistry |
| Biochem/physiol Actions: | Antibodies reacting specifically with Annexin VII serve as an essential tool for the detection of this molecule in various cells. The human annexin VII (ANXA7) possesses a tumor suppressor role in prostate cancer. Hence it can be used an important biomarker along with syndecan-1 to predict the survival rates in prostate cancer patients. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Annexin VII (synexin) is a membrane binding protein that localizes in the nucleus and cytosol. In humans and mice, Annexin VII is encoded by a single gene that has alternatively spliced cassette exons. As a result, two isoforms of molecular mass of 47kDa and 51kDa are expressed. These isoforms differ in their N-terminal region and also have distinct tissue-specific expression patterns. |
| General description: | Anti-Annexin VII antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 203-217/6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant mouse Annexin VII. The human annexin VII (ANXA7) is located on chromosome 10q21. |
| Immunogen: | recombinant mouse annexin VII. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| Specificity: | Mouse monoclonal anti-annexin VII antibody reacts with human, bovine, rabbit, dog, rat, mouse, and chicken Annexin VII. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |