Anti-ASPP2 antibody, Mouse monoclonal
SIGMA/A4480 - ~1.5 mg/mL, clone DX 54.10, purified from hybridoma cell culture
Synonym: Anti-53BP2 apoptosis-
MDL Number: MFCD08705575
Product Type: Chemical
Western Blotting Whole extract of U-2-OS cells were separated on SDS-PAGE and probed with Monoclonal Anti-ASPP2 antibody produced in Mouse, Clone: DX 54.10 (Cat. No. A4480). The antibody was developed using 1:5,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A2304). Lanes 1. 1:1,000 antibody 2. 1:2,000 antibody 3. 1:4,000 antibody 4. 1:8,000 antibody 5. Negative Control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | DX 54.10, monoclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~175 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| western blot: 0.5-1 μg/mL using total cell extract of human osteogenic sarcoma | |
| UniProt accession no. | Q13625  |
| Application: | Mouse Monoclonal Anti-ASPP2 has been used for immunofluorescence analysis. The antibody can also be used for immunocytochemistry, immunoprecipitation and microarray applications. |
| Application: | Western blot analysis of Hek293 or 3T3-L1 adipocyte cell lysates was performed using monoclonal mouse anti-ASPP2 as the primary. |
| Biochem/physiol Actions: | Apoptosis-stimulating protein of p53 (ASPP2) enhances the ability of p53 to induce apoptosis ASPP1 and ASPP2 stimulates the transactivation function of p63 and p73 on promoters of different genes such as BCL2 associated X, apoptosis regulator (Bax), p53-inducible gene 3 (PIG3) and p53 upregulated modulator of apoptosis (PUMA), but not mouse double minute 2 homolog (Mdm2) or p21WAF-1/CIP1. |
| Biochem/physiol Actions: | ASPP2 is a tumor suppressor that activates p53. ASPP2 also regulates Par-3 which subsequently modulates the proliferation and polarity of neural progenitor cells during CNS development. Furthermore, ASPP2 interacts with active Ras to induce p53 mediated cell death. ASPP2 is known to function as a biomarker for invasive pituitary adenomas. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-ASPP2 (mouse IgG1 isotype) is derived from the hybridoma DX 54.10 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice. Apoptosis-stimulating protein of p53 (ASPP) family of proteins contains three members, ASPP1, ASPP2 and iASPP, which interact via their C-terminal region with p53 and modulate its activity. |
| Immunogen: | fragment of human ASPP2 (amino acids 691-1128). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Monoclonal Anti-ASPP2 recognizes human and mouse ASPP2. The epitope recognized by the antibody resides within amino acids 691-1128 of human ASPP2. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |