Bovine Serum Albumin
SIGMA/A4503 - cold ethanol fraction, pH 5.2, ≥96%
Synonym: Albumin bovine serum; BSA; Bovine albumin
CAS Number: 9048-46-8
EC Number: 232-936-2
MDL Number: MFCD00130384
Product Type: Chemical
assay | ≥96% |
biological source | bovine |
foreign activity | BT Virus, none detected |
VSV Virus, none detected | |
form | lyophilized powder |
loss | ≤5% |
mol wt | ~66 kDa |
origin | USA origin |
pH | 5.2 |
purified by | cold ethanol fractionation |
Quality Level | 300 |
solubility | water: soluble (40 mg/ml) |
storage temp. | 2-8°C |
technique(s) | blood typing: suitable |
ELISA: suitable | |
microbiological culture: suitable | |
UniProt accession no. | P02769 |
Application: | • Bovine Serum Albumin has been used in solution prepared for the isolation of heart mitochondria. • It has been used in the cytotoxicity assay buffer. • It has been used for the induction of protein overload nephropathy in rats. |
Biochem/physiol Actions: | Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies. |
General description: | Bovine serum albumin is a model protein in various studies and is made up of 583 amino acids. It is a water-soluble protein with a molecular weight of 66.4kDa. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating. |
Packaging: | 1, 5 kg in poly drum |
Packaging: | 10, 50, 100, 500 g in poly bottle |
Preparation Note: | Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method. |
Preparation Note: | Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥96% |
Storage Temp. | 2-8°C |
UNSPSC | 12352202 |